Department of Parasitology, Faculty of Veterinary Medicine University of Firat, 23119 Elazig, Turkey.
Vet Parasitol. 2011 Dec 29;183(1-2):109-13. doi: 10.1016/j.vetpar.2011.06.012. Epub 2011 Jun 24.
The prevalence of Dirofilaria immitis infection was evaluated in stray dogs of Erzurum, Turkey. A total of 123 whole-blood and 93 sera samples were collected from stray dogs older than 6 months were lived in animal shelter. The PCR and direct microscopic examinations were used for the detection of microfilaria and indirect-ELISA was performed for the detection of anti-D. immitis antibodies. The prevalence of D. immitis in the canine population was 8.1% by PCR, 2.1% by ELISA. In addition, microfilaria burdens of Dirofilaria sp. was 4.8% by direct blood smear examination. There was a statistical difference (P=0.05) in the prevalence between males (10.5%) and females (2.3%) by direct blood smear examination. Similarly there was a statistical difference (P<0.05) in the prevalence between males (15.8%) and females (4.7%) by PCR. Dogs belonging to the 0.5-1 years old group showed the highest prevalence than 2-4 ages group with three tests. Among the 93 samples screened by the ELISA, two samples were positive for the D. immitis antibodies. Both positive dogs with ELISA were females.
本研究旨在评估土耳其埃尔祖鲁姆地区流浪犬感染犬恶丝虫的流行情况。共采集了 123 份全血样本和 93 份血清样本，这些样本均来自年龄大于 6 个月、生活在动物收容所的流浪犬。采用 PCR 和直接显微镜检查法检测微丝蚴，间接酶联免疫吸附试验（ELISA）检测抗犬恶丝虫抗体。PCR 检测犬恶丝虫的流行率为 8.1%，ELISA 检测为 2.1%。此外，直接血涂片检查发现犬恶丝虫的微丝蚴负荷率为 4.8%。直接血涂片检查发现雄性（10.5%）和雌性（2.3%）的流行率存在统计学差异（P=0.05）。类似地，PCR 检测发现雄性（15.8%）和雌性（4.7%）的流行率也存在统计学差异（P<0.05）。在三个检测中，0.5-1 岁年龄组的犬的感染率最高，2-4 岁年龄组的犬感染率最低。在 93 份经 ELISA 筛选的样本中，有 2 份样本的犬恶丝虫抗体呈阳性。这两只 ELISA 阳性的犬均为雌性。
