Baram G I, Grachev M A, Malikov N G, Nazimov P V, Shemiakin V V
Bioorg Khim. 1991 Sep;17(9):1166-71.
The primary structure of myoglobin of the seal of Lake Baikal (East Siberia) Phoca siberica, determined by sequencing the whole protein and peptides obtained by the cyanogen bromide or proteinase cleavage and separated by the microcolumn liquid chromatography, was found to be identical to the primary structures of myoglobins of the harbour seal Phoca vitulina largha and the grey seal Halehoerus gryphus. It suggests that these species separated from a common ancestor less than seven million years ago.
通过对贝加尔湖海豹(东西伯利亚)即西伯利亚海豹的肌红蛋白进行全蛋白测序以及对经溴化氰或蛋白酶裂解获得并通过微柱液相色谱分离的肽段进行测序,确定其一级结构与港海豹(髯海豹)和灰海豹的肌红蛋白一级结构相同。这表明这些物种在不到七百万年前从一个共同祖先分化而来。