Kumar Ravindra, Singh Poonam Jayant, Nagpure N S, Kushwaha Basdeo, Srivastava S K, Lakra W S
National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, India.
Indian J Exp Biol. 2007 Nov;45(11):992-7.
DNA markers are being increasingly used in studies related to population genetics and conservation biology of endangered species. DNA isolation for such studies requires a source of biological material that is easy to collect, non-bulky and reliable. Further, the sampling strategies based on non-invasive procedures are desirable, especially for the endangered fish species. In view of above, a rapid DNA extraction method from fish scales has been developed with the use of a modified lysis buffer that require about 2 hr duration. This methodology is non-invasive, less expensive and reproducible with high efficiency of DNA recovery. The DNA extracted by this technique, have been found suitable for performing restriction enzyme digestion and PCR amplification. Therefore, the present DNA extraction procedure can be used as an alternative technique in population genetic studies pertaining to endangered fish species. The technique was also found equally effective for DNA isolation from fresh, dried and ethanol preserved scales.
DNA标记在与濒危物种的群体遗传学和保护生物学相关的研究中越来越多地被使用。此类研究的DNA分离需要易于收集、体积不大且可靠的生物材料来源。此外，基于非侵入性程序的采样策略是可取的，特别是对于濒危鱼类物种。鉴于上述情况，已开发出一种从鱼鳞中快速提取DNA的方法，该方法使用了一种改良的裂解缓冲液，耗时约2小时。该方法是非侵入性的，成本较低且可重复，DNA回收率高。通过该技术提取的DNA已被发现适用于进行限制性酶切消化和PCR扩增。因此，目前的DNA提取程序可作为濒危鱼类物种群体遗传学研究中的一种替代技术。该技术在从新鲜、干燥和乙醇保存的鳞片中分离DNA方面也同样有效。
