Harangi Mariann, Seres Ildiko, Magyar Maria T, Csipo Istvan, Sipka Sandor, Valikovics Attila, Csiba Laszlo, Bereczki Daniel, Paragh Gyorgy
1st Department of Medicine, University of Debrecen, Debrecen, Hungary.
Cerebrovasc Dis. 2008;25(1-2):122-8. doi: 10.1159/000112322. Epub 2007 Dec 11.
Human serum paraoxonase (PON1) protects lipoproteins against oxidation by hydrolyzing lipid peroxides in oxidized low-density lipoprotein (oxLDL); therefore, it may protect against atherosclerosis. PON1 activity and polymorphisms have been inconsistently associated with carotid artery disease. The goal of this study was to clarify the role of PON1 activity and phenotype on carotid artery disease and its correlation with some inflammatory and immune markers in subjects under 55 years with early-onset carotid atherosclerosis.
Sixty patients with occlusive carotid artery disease and 30 healthy controls were enrolled. Intima-media thickness (IMT) was measured by high-resolution ultrasound of both common carotid arteries. Anti-oxLDL antibody levels were determined by ELISA.
In the whole study population we found a negative correlation between PON1 activity and IMT (r = -0.27, p = 0.011), and between salt-stimulated PON1 activity and IMT (r = -0.24, p = 0.02). Both PON1 activity and salt-stimulated PON1 activity negatively correlated with anti-oxLDL levels (r = -0.28, p = 0.008; r = -0.26, p = 0.01). PON1 activity was lower in patients compared to controls; however, the difference was not significant.PON1 phenotype distribution of patients and controls did not differ significantly.
The importance of PON1 activity as a predictive risk factor for early-onset occlusive carotid artery disease should be assessed in future studies.
人血清对氧磷酶（PON1）通过水解氧化型低密度脂蛋白（oxLDL）中的脂质过氧化物来保护脂蛋白免受氧化；因此，它可能预防动脉粥样硬化。PON1活性和多态性与颈动脉疾病的关联并不一致。本研究的目的是阐明PON1活性和表型在早发性颈动脉粥样硬化的55岁以下受试者中对颈动脉疾病的作用及其与一些炎症和免疫标志物的相关性。
招募了60例患有闭塞性颈动脉疾病的患者和30名健康对照。通过对双侧颈总动脉进行高分辨率超声测量内膜中层厚度（IMT）。采用酶联免疫吸附测定法测定抗oxLDL抗体水平。
在整个研究人群中，我们发现PON1活性与IMT之间呈负相关（r = -0.27，p = 0.011），盐刺激的PON1活性与IMT之间也呈负相关（r = -0.24，p = 0.02）。PON1活性和盐刺激的PON1活性均与抗oxLDL水平呈负相关（r = -0.28，p = 0.008；r = -0.26，p = 0.01）。患者的PON1活性低于对照组；然而，差异不显著。患者和对照组的PON1表型分布没有显著差异。
未来的研究应评估PON1活性作为早发性闭塞性颈动脉疾病预测危险因素的重要性。
