Correa Fernando, Docagne Fabian, Mestre Leyre, Loría Frida, Hernangómez Miriam, Borrell José, Guaza Carmen
Neuroimmunology Group, Functional and Systems Neurobiology Department, Instituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Neuroimmunomodulation. 2007;14(3-4):182-7. doi: 10.1159/000110644. Epub 2007 Dec 5.
There is a growing amount of evidence suggesting that cannabinoids may be neuroprotective in central nervous system inflammatory conditions. Advances in the understanding of the physiology and pharmacology of the cannabinoid system have potentiated the interest in cannabinoids as potential therapeutic targets. Here our aim was to update the actions of cannabinoids on immune system and glial cells and their implications on multiple sclerosis. We also show our results on the modulation of cytokines of the IL-12 family by cannabinoids in macrophages and brain microglia. We used murine primary cultures of macrophage and microglia activated by lipopolysaccharide/IFN-gamma and Theiler's virus to study the effects of cannabinoids on the regulation of IL-12 and IL-23 mRNA and protein IL-12p40, evaluated by RT-PCR and ELISA, respectively. Cannabinoids negatively regulate the production of these cytokines by microglial cells in part due to the activation of CB(2) receptors. The effects of cannabinoids on cytokine brain work and on the regulation of neuroinflammatory processes may affect chronic inflammatory demyelinating diseases such as multiple sclerosis.
越来越多的证据表明，大麻素在中枢神经系统炎症性疾病中可能具有神经保护作用。对大麻素系统生理学和药理学认识的进展增强了人们对大麻素作为潜在治疗靶点的兴趣。在此，我们的目的是更新大麻素对免疫系统和神经胶质细胞的作用及其对多发性硬化症的影响。我们还展示了大麻素对巨噬细胞和脑小胶质细胞中IL-12家族细胞因子的调节作用的研究结果。我们使用经脂多糖/γ干扰素和泰勒氏病毒激活的小鼠巨噬细胞和小胶质细胞原代培养物，分别通过RT-PCR和ELISA评估大麻素对IL-12和IL-23 mRNA以及蛋白IL-12p40表达的调节作用。大麻素通过激活CB(2)受体部分地负向调节小胶质细胞中这些细胞因子的产生。大麻素对细胞因子在脑内的作用以及对神经炎症过程的调节作用可能会影响诸如多发性硬化症等慢性炎症性脱髓鞘疾病。
