Zhi-ming Lu, Yu-lian Jiao, Zhao-lei Feng, Chun-xiao Wu, Zhen-fang Dong, Bing-chang Zhang, Yue-ran Zhao
Department of Center Laboratory, Shandong Provincial Hospital, Shandong University, 324 Jing Wu Rd 250021 Jinan, China.
Croat Med J. 2007 Dec;48(6):800-6. doi: 10.3325/cmj.2007.6.800.
To explore whether killer cell immunoglobulin-like receptors (KIR) gene polymorphisms are associated with susceptibility to persistent hepatitis B virus (HBV) infection or HBV clearance.
Fifteen known KIR genes were determined in 150 chronic hepatitis B patients, 251 spontaneously recovered controls, and 412 healthy controls by the sequence specific primer polymerase chain reaction (SSP-PCR) method. KIR genotype frequency (gf) differences were tested for significance by two-tailed Fisher exact test or chi(2) test. Multifactorial analysis was also performed by logistic analysis (the SAS system).
Framework genes KIR2DL4, KIR3DL2, KIR3DL3, and KIRZ were present in all individuals. The frequencies of KIR2DS2 and KIR2DS3 were higher in chronic hepatitis B patients, than in both healthy and spontaneously recovered controls. The frequencies of activating KIR2DS1, KIR3DS1, and the inhibitory KIR2DL5 were higher in spontaneously recovered controls than in chronic hepatitis B patients and healthy controls.
KIR polymorphisms may be associated with susceptibility to HBV infection or HBV clearance. It could be suggested that KIR2DS2 and KIR2DS3 were HBV-susceptive genes, which induced a persistent yet weak inflammatory reaction that resulted in continuous injury of live tissues and chronic hepatitis. KIR2DS1, KIR3DS1, and KIR2DL5, on the other hand, may be protective genes that facilitated the clearance of HBV.
探讨杀伤细胞免疫球蛋白样受体（KIR）基因多态性与持续性乙型肝炎病毒（HBV）感染易感性或HBV清除之间的关联。
采用序列特异性引物聚合酶链反应（SSP-PCR）方法，对150例慢性乙型肝炎患者、251例自发康复对照者和412例健康对照者的15个已知KIR基因进行检测。通过双侧Fisher精确检验或卡方检验对KIR基因型频率（gf）差异进行显著性检验。还通过逻辑分析（SAS系统）进行多因素分析。
框架基因KIR2DL4、KIR3DL2、KIR3DL3和KIRZ在所有个体中均存在。慢性乙型肝炎患者中KIR2DS2和KIR2DS3的频率高于健康对照者和自发康复对照者。自发康复对照者中激活型KIR2DS1、KIR3DS1和抑制型KIR2DL5的频率高于慢性乙型肝炎患者和健康对照者。
KIR基因多态性可能与HBV感染易感性或HBV清除有关。可以认为KIR2DS2和KIR2DS3是HBV易感基因，它们诱导持续但微弱的炎症反应，导致肝组织持续损伤和慢性肝炎。另一方面，KIR2DS1、KIR3DS1和KIR2DL5可能是促进HBV清除的保护基因。
