Yan L-X, Zhu F-M, Jiang K, He J
Key Laboratory of Blood Safety Research of Ministry of Health, Wulin Road 345, Hangzhou, Zhejiang Province 310006, China.
Tissue Antigens. 2007 Feb;69(2):133-8. doi: 10.1111/j.1399-0039.2006.00746.x.
Human killer cell immunoglobulin-like receptors are a subfamily of the immunoglobulin superfamily, which map to the leukocyte receptor complex on chromosome 19. Here, we established polymerase chain reaction-sequence-based typing (PCR-SBT) procedures to identify alleles of the KIR2DS4 gene. The method was designed around the specific amplification of exons 4-5 of the KIR2DS4 gene. Genomic DNA from 105 healthy unrelated Chinese Han individuals were typed for the KIR2DS4 alleles. Each sample was assigned the KIR2DS4 alleles combination, consistent with the pairwise combinations of sequences of all the known KIR2DS4 alleles. We observed eleven different genotypes and four KIR2DS4 alleles in the population, with the KIR2DS400101 having the highest frequency, 0.576, and also confirmed the new KIR2DS4007 allele. Our data demonstrated that the established PCR-SBT method for the KIR2DS4 allele typing was reliable.
人类杀伤细胞免疫球蛋白样受体是免疫球蛋白超家族的一个亚家族，定位于19号染色体上的白细胞受体复合体。在此，我们建立了基于聚合酶链反应-序列分型（PCR-SBT）的方法来鉴定KIR2DS4基因的等位基因。该方法围绕KIR2DS4基因外显子4-5的特异性扩增设计。对105名健康无亲缘关系的中国汉族个体的基因组DNA进行KIR2DS4等位基因分型。每个样本都被指定了KIR2DS4等位基因组合，与所有已知KIR2DS4等位基因序列的两两组合一致。我们在该人群中观察到11种不同的基因型和4个KIR2DS4等位基因，其中KIR2DS400101频率最高，为0.576，同时还证实了新的KIR2DS4007等位基因。我们的数据表明，所建立的用于KIR2DS4等位基因分型的PCR-SBT方法是可靠的。
