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[用电刺激恢复因体温逐渐降低而停搏且未进行心脏复温的离体心脏的收缩]

[Restoration of contractions of isolated heart arrested in result of progressing hypothermia with electrical stimuli and no warming up of the heart].

作者信息

Aliukhin Iu S

出版信息

Ross Fiziol Zh Im I M Sechenova. 2007 Oct;93(10):1181-7.

Abstract

In the rat isolated heart, the effect of cooling with the velocity 0.2 degrees C/min and its stimulation with rectangle electrical pulses (19 Hz, 100 ms, 1-5 V amplitude) on the heart mechanical activity in mcWatt/g was studied as the product of heart shortening on contraction strength and heart rate. The heart shortening was measured by the Langendorf method. Heart contractions activated the recording system with the aid of a hook at the apex. The Ringer-Lokk solution served as perfusate pumped with peristaltic pump. After cold arrest of automatic contractions (on the average at 9.3 +/- 0.7 degrees C), the heart continued to respond with contractions to electrical pulses of 5 V amplitude, the power of the heart contractions at 10 degrees C being on the average 35 % of the initial power at 20 degrees C. Excitability and contractility of the heart terminate at 5.3 +/- 0.4 degrees C.

摘要

在大鼠离体心脏实验中,研究了以0.2℃/min的速度进行冷却以及用矩形电脉冲(19Hz,100ms,幅度1 - 5V)刺激对心脏机械活动的影响,心脏机械活动以每克心肌瓦特(mcWatt/g)为单位,通过心脏缩短幅度与收缩强度及心率的乘积来衡量。心脏缩短幅度采用Langendorf法测量。心脏收缩通过心尖处的挂钩激活记录系统。用蠕动泵泵入任氏-洛克溶液作为灌注液。在自动收缩冷停搏后(平均温度为9.3±0.7℃),心脏对幅度为5V的电脉冲仍继续产生收缩反应,10℃时心脏收缩功率平均为20℃时初始功率的35%。心脏的兴奋性和收缩性在5.3±0.4℃时终止。

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