水蛭乙醇提取物体外抗凝活性的研究
[Study of anticoagulant activity of ethanol extracts from leech in vitro].
作者信息
Feng Guang-jun, Zhu Zheng-guang, Yu Chuan-lin, Zhang Qun, Chen Na-na, Lei Lin-sheng, Wu Shu-guang
机构信息
Institute of Pharmaceutical Science, College of Pharmaceutical Science, Southern Medical University, Guangzhou 510515, China.
出版信息
Zhong Yao Cai. 2007 Aug;30(8):909-11.
OBJECTIVE
To study the anticoagulant activity of different portions of leech ethanol extracts (LEEs).
METHODS
Anticoagulant activity was determined by measuring prothrombin time(PT), thrombin time (TT), activated partial throboplstin time (APTT) and fibrinogen coagulation time (FCT).
RESULTS
PT, TT, APTT and FCT were remarkably prolonged by ethyl acetate portion of LEEs. Portions of petroleum ethrer, n-butanol and water extracted from LEEs was much weaker on anticoagulant activity.
CONCLUSION
The anticoagulant effect of ethyl acetate extract portion of LEEs is the strongest among the four portions, and this results may be from its inhibitory effect on thrombin-catalyzed fibrinogen hydrolysis.
目的
研究水蛭乙醇提取物（LEEs）不同部位的抗凝活性。
方法
通过测定凝血酶原时间（PT）、凝血酶时间（TT）、活化部分凝血活酶时间（APTT）和纤维蛋白原凝固时间（FCT）来确定抗凝活性。
结果
LEEs的乙酸乙酯部位显著延长PT、TT、APTT和FCT。LEEs的石油醚、正丁醇和水提取部位的抗凝活性较弱得多。
结论
LEEs的乙酸乙酯提取物部位的抗凝作用在四个部位中最强，这一结果可能源于其对凝血酶催化的纤维蛋白原水解的抑制作用。
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