水蛭不同提取物体外抗凝活性的实验研究
[Experimental studies on anticoagulant activity of different extract of leech in vitro].
作者信息
Li Yanling, Huang Rongqing, Sun Xiaodong
机构信息
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850.
出版信息
Zhong Yao Cai. 2004 Feb;27(2):123-5.
OBJECTIVE
To study the anticoagulant activity of different extracts of Leech.
METHODS
Prothrombin time (PT), thrombin time (TT) and activated partial thromboplastin time (APTT) were determined by coagulometer for blood samples collected from abdominal vein of Wistar rat.
RESULTS
PT, TT, APTT were prolonged remarkably by ethyl acetate part of Leech. PT was prolonged remarkably by hexane part and aqueous solution part of Leech. APTT was prolonged remarkably by n-Butylalcohol part of Leech.
CONCLUSION
The different extracts of Leech have obvious anticoagulant effects. There are new anticoagulant components different from hirudin in Leech.
目的
研究水蛭不同提取物的抗凝活性。
方法
采用凝血仪测定从Wistar大鼠腹静脉采集的血液样本的凝血酶原时间（PT）、凝血酶时间（TT）和活化部分凝血活酶时间（APTT）。
结果
水蛭乙酸乙酯部位可显著延长PT、TT、APTT。水蛭正己烷部位和水溶液部位可显著延长PT。水蛭正丁醇部位可显著延长APTT。
结论
水蛭不同提取物具有明显的抗凝作用。水蛭中存在不同于水蛭素的新型抗凝成分。
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