[毛细管电泳法分离测定山茱萸中齐墩果酸和熊果酸]

[Separation and determination of oleanolic acid and ursolic acid from Cornus officinalis by capillary electrophoresis].

作者信息

Wang Rui, Wang Shu-mei, Liang Sheng-wang, Xu Guo-wang, Weng Qian-feng

机构信息

Beijing University of Chinese Medicine, Beijing 100102, China.

出版信息

Zhong Yao Cai. 2007 Aug;30(8):946-50.

PMID:18074841

Abstract

A cycledextrin-modified micellar electrokinetic chromatography technique for separating and determination oleanolic acid and ursolic acid in the fruit of Cornus officinalis was developed. The two bio-active components was completely separated using a buffer containing 60 mmol/L SDS, 2.5 mmol/L NaH2PO4, 2.5 mmol/L Na2B4O7, 8 mmol/L DM beta-CD,8 mmol/L beta-CD and 30% (v/v) 2-propanol. The correlation cofficients of the linear calibration graphs for oleanolic acid and ursolic acid are 0. 9996 and 0.9995, respectively. The effects of SDS, 2-propanol, dimethyl-beta-cyclodextrin, beta-cyclodextrin, NaH2PO4, Na2B4O7 and pH on separation were investigated.

摘要

建立了一种用于分离和测定山茱萸果实中齐墩果酸和熊果酸的环糊精修饰胶束电动色谱技术。使用含有60 mmol/L十二烷基硫酸钠（SDS）、2.5 mmol/L磷酸二氢钠、2.5 mmol/L硼砂、8 mmol/L二甲基-β-环糊精、8 mmol/L β-环糊精和30%（v/v）异丙醇的缓冲液，可将这两种生物活性成分完全分离。齐墩果酸和熊果酸线性校准曲线的相关系数分别为0.9996和0.9995。研究了SDS、异丙醇、二甲基-β-环糊精、β-环糊精、磷酸二氢钠、硼砂和pH对分离的影响。