Kaznowska Ewa, Przybyłowska Karolina, Kulig Andrzej
Tuberculosis and Pulmonary Diseases Specialistic Hospital in Rzeszów.
Pol J Pathol. 2007;58(3):189-92.
Hamartomas are the third most common cause of solitary pulmonary nodule and the most common benign tumors of lung. Recent study indicated that hamartoma may be associated with a chronic inflammatory diseases. Histochemical analysis of the expression profile of growth-relevant was shown the upregulation of macrophage migration inhibitory factor (MIF) in hamartomas and surrounding lung parenchyma. We investigated polymorphism G/C at position -173 promoter gene of MIF, pro-inflammatory cytokine in pulmonary hamartoma. This polymorphism of the MIF gene are association with increased production of MIF and have been found to confer increased risk of susceptibility to chronic inflammatory diseases. DNA samples were obtained from hamartoma tissue fixed with formalin, embedded in paraffin, from 52 patients and from blood samples of 123 sex and age matched healthy person served as control. The G/C polymorphism of MIF gene was determined by PCR-based AluI restriction fragment length polimorphism. The frequencies of the C allele did not differ significantly between pulmonary hamartoma patients and healthy controls (18% vs 15%, OR 1.26 CI95% 0.68-2.40). The obtained results suggest no association between G/C polymorphism at promoter gene of MIF and the incidence of pulmonary hamartoma, but our study has a preliminary character and should be extended on larger population.
错构瘤是孤立性肺结节的第三大常见病因，也是肺部最常见的良性肿瘤。最近的研究表明，错构瘤可能与慢性炎症性疾病有关。对生长相关表达谱的组织化学分析显示，错构瘤及周围肺实质中巨噬细胞移动抑制因子（MIF）上调。我们研究了肺部错构瘤中促炎细胞因子MIF启动子基因-173位的G/C多态性。MIF基因的这种多态性与MIF产生增加有关，并且已发现其会增加患慢性炎症性疾病的易感性风险。DNA样本取自52例患者用福尔马林固定、石蜡包埋的错构瘤组织，以及123名年龄和性别匹配的健康人的血液样本作为对照。MIF基因的G/C多态性通过基于PCR的AluI限制性片段长度多态性来确定。错构瘤患者和健康对照之间C等位基因的频率没有显著差异（18%对15%，OR 1.26，CI95% 0.68 - 2.40）。所得结果表明MIF启动子基因的G/C多态性与肺部错构瘤的发病率之间没有关联，但我们的研究具有初步性质，应在更大规模人群中进行扩展研究。
