将jog基因座定位到小鼠6号染色体上D6Mit104和D6Mit336之间的区域。

Mapping of jog locus to the region between D6Mit104 and D6Mit336 on mouse chromosome 6.

作者信息

Sun Xiao-yang, Chen Zi-yan, Kanou Yasuhiko, Takagishi Yoshiko, Hayashi Yoshitaka, Ohno Tamio, Murata Yoshiharu, Oda Sen-ichi

机构信息

Department of Genetics, Division of Stress Adaptation and Protection, Research Institute of Environmental Medicine, Nagoya University, Japan.

出版信息

Exp Anim. 2007 Oct;56(5):389-92. doi: 10.1538/expanim.56.389.

DOI:10.1538/expanim.56.389

PMID:18075201

Abstract

The joggle mouse is a recessive ataxic mutant carrying an unknown mutation in a C3H/He (C3H)-derived chromosomal segment. Taking advantage of the mouse genome database, we selected 127 DNA microsatellite markers showing heterozygosity between C3H and C57BL/6J (B6) and a first round of screening for the joggle mutation was performed on B6-jog/+ partial congenic mice (N4). We identified 4 chromosomal regions in which 13 microsatellite markers show heterozygosity between C3H and B6. Then, we analyzed the genotype of these 4 chromosomal regions in mice that showed the joggle phenotype and mapped the jog locus between markers D6Mit104 (111.4 Mb) and D6Mit336 (125.1 Mb) (an interval of 13.7 Mb) on chromosome 6. By using a partial congenic strain together with the mouse genome database, we successfully mapped the chromosomal localization of the jog locus much more efficiently than by conventional linkage analysis.

摘要

轻摇小鼠是一种隐性共济失调突变体，在源自C3H/He（C3H）的染色体片段中携带一个未知突变。利用小鼠基因组数据库，我们选择了127个在C3H和C57BL/6J（B6）之间显示杂合性的DNA微卫星标记，并对B6-jog/+部分同源基因小鼠（N4）进行了第一轮轻摇突变筛选。我们确定了4个染色体区域，其中13个微卫星标记在C3H和B6之间显示杂合性。然后，我们分析了表现出轻摇表型的小鼠中这4个染色体区域的基因型，并将轻摇基因座定位在6号染色体上的标记D6Mit104（111.4 Mb）和D6Mit336（125.1 Mb）之间（间隔为13.7 Mb）。通过使用部分同源基因品系和小鼠基因组数据库，我们比通过传统连锁分析更有效地成功定位了轻摇基因座的染色体位置。