Nilsson B O, Lindh U, Sunde T, Wide M
Department of Human Anatomy, Biomedical Centre, Uppsala, Sweden.
Reprod Toxicol. 1991;5(1):53-5. doi: 10.1016/0890-6238(91)90110-2.
Mice were injected intravenously with lead chloride, 75 micrograms/g body weight. The mice were in an experimental delay of implantation, which offered the functionally steady conditions required for developing the testing procedures. One day after the administration of lead, blastocysts were flushed from the uterine cavity, placed on thin foils of Kapton, and air-dried. The dried blastocysts were analyzed in a nuclear microprobe with a particle intensity of 10 nanoampere (nA) for 300 s using a spatial resolution of 3 microns. The average lead concentration of blastocysts at implantation was 3.90 micrograms/g dry weight. We judge this technique to be useful for evaluating the transport of heavy metals from mother to preembryos, not only under the present experimental conditions but also in normal pregnancies and in other species.
给小鼠静脉注射氯化铅，剂量为75微克/克体重。这些小鼠处于植入实验性延迟状态，这为开发测试程序提供了所需的功能稳定条件。给予铅一天后，从子宫腔冲洗出胚泡，置于聚酰亚胺薄箔上并风干。使用空间分辨率为3微米的核微探针，以10纳安（nA）的粒子强度对干燥的胚泡进行300秒分析。植入时胚泡的平均铅浓度为3.90微克/克干重。我们认为该技术不仅在当前实验条件下，而且在正常妊娠和其他物种中，对于评估重金属从母体到胚胎前期的转运都是有用的。
