对意大利南部卡拉布里亚地区特有的植物巴氏碎米荠（十字花科）自由基的影响及对α-淀粉酶的抑制作用

Effects on free radicals and inhibition of alpha-amylase of Cardamine battagliae (Cruciferae), an apoendemic Calabrian (southern Italy) plant.

作者信息

Conforti F, Vaccaro A, Loizzo M R, Statti G A, Autelitano G, Menichini F

机构信息

Department of Pharmaceutical Sciences, University of Calabria, Rende (CS), Italy.

出版信息

Nat Prod Res. 2008 Jan 20;22(2):101-7. doi: 10.1080/14786410600885612.

DOI:10.1080/14786410600885612

PMID:18075893

Abstract

The present study showed for the first time the biological properties of different fractions from Cardamine battagliae Cesca & Peruzzi, an apoendemic Calabrian (southern Italy) plant that belongs to the Cruciferae family. The antioxidant activities of the different fractions of C. battagliae were carried out using two different in vitro assays (beta-carotene bleaching test and lipid peroxidation of liposomes assay) while radical scavenging activity was carried out using DPPH test. AcOEt fraction showed the highest activity on DPPH inhibition (IC50 of 0.162 mg mL(-1)) while dichloromethane fraction showed the highest activity on beta-carotene bleaching (IC(50) of 0.004 mg mL(-1)). The assay for alpha-amylase inhibition showed that n-hexane fraction showed the highest activity with an IC50 of 0.055 mg mL(-1).

摘要

本研究首次展示了碎米荠（Cardamine battagliae Cesca & Peruzzi）不同提取物的生物学特性，碎米荠是一种意大利南部卡拉布里亚地区特有的十字花科植物。采用两种不同的体外试验（β-胡萝卜素漂白试验和脂质体脂质过氧化试验）对碎米荠不同提取物的抗氧化活性进行了检测，同时采用DPPH试验检测了其自由基清除活性。乙酸乙酯提取物对DPPH抑制活性最高（IC50为0.162 mg mL(-1)），而二氯甲烷提取物对β-胡萝卜素漂白活性最高（IC50为0.004 mg mL(-1)）。α-淀粉酶抑制试验表明，正己烷提取物活性最高，IC50为0.055 mg mL(-1)。