黑鳗藤抗氧化及清除自由基特性的评估

Evaluation of the antioxidant and free radical scavenging properties of Secamone afzelii Rhoem.

作者信息

Mensah A Y, Houghton P J, Akyirem G N A, Fleischer T C, Mensah M L K, Sarpong K, Adosraku R

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

出版信息

Phytother Res. 2004 Dec;18(12):1031-2. doi: 10.1002/ptr.1614.

DOI:10.1002/ptr.1614

PMID:15742341

Abstract

The antioxidant activity of a methanol extract of Secamone afzelii stems was tested using the DPPH assay and the active compound was identified as alpha-tocopherol. HPLC determination showed that 0.12% w/w alpha-tocopherol was present in the plant. The total extract also showed effective free radical scavenging activity in the assay for non-enzymatic lipid peroxidation in liposomes with an IC50 value of 90 microg/mL, with alpha-tocopherol isolated from the plant having an IC50 of 15 microg/mL in the same system, thus demonstrating the presence of other antioxidants.

摘要

采用DPPH法对非洲鲫鱼藤茎甲醇提取物的抗氧化活性进行了测试，活性化合物被鉴定为α-生育酚。高效液相色谱测定表明，该植物中含有0.12%（w/w）的α-生育酚。在脂质体非酶促脂质过氧化试验中，总提取物也表现出有效的自由基清除活性，IC50值为90μg/mL，从该植物中分离出的α-生育酚在同一系统中的IC50为15μg/mL，从而证明存在其他抗氧化剂。