Marol Serhat, Yücesoy Mine
Department of Microbiology and Clinical Microbiology, School of Medicine, Dokuz Eylül University, Inciralti, Izmir, Turkey.
Mycoses. 2008 Jan;51(1):40-9. doi: 10.1111/j.1439-0507.2007.01435.x.
Epidemiological analysis of nosocomial Candida infections has gained importance due to an increase in these infections during the recent years. This study investigated the prevalence of clinical infections of Candida in anesthesiology intensive care unit patients, and ascertains the level of genetic diversity in the Candida species. A total of 70 Candida isolates, consisting of 42 Candida albicans, 16 Candida glabrata and 12 Candida tropicalis strains isolated from various clinical sites of infection of anesthesiology intensive care unit patients, were analysed. The susceptibility of the isolates against amphotericin B and fluconazole was determined by microdilution method according to Clinical and Laboratory Standards Institute M27-A2 standards. The strains were typed by random amplified polymorphic DNA (RAPD)-PCR using OPE-03, OPE-18, RP4-2 and AP50-1 primers. In the patients with Candida infections, most isolates exhibited different RAPD patterns. Only three C. albicans pairs isolated within a short time period had the same RAPD pattern. Most of the Candida infections in the anesthesiology intensive care unit of our hospital seem to be caused by endogenous strains. Exogenous spread of C. albicans infections occurred less frequently.
近年来，医院内念珠菌感染有所增加，因此对其进行流行病学分析变得愈发重要。本研究调查了麻醉重症监护病房患者念珠菌临床感染的患病率，并确定了念珠菌属的遗传多样性水平。对从麻醉重症监护病房患者的不同临床感染部位分离出的70株念珠菌进行了分析，其中包括42株白色念珠菌、16株光滑念珠菌和12株热带念珠菌。根据临床和实验室标准协会M27 - A2标准，采用微量稀释法测定分离株对两性霉素B和氟康唑的敏感性。使用OPE - 03、OPE - 18、RP4 - 2和AP50 - 1引物通过随机扩增多态性DNA（RAPD）-PCR对菌株进行分型。在念珠菌感染患者中，大多数分离株呈现不同的RAPD模式。在短时间内分离出的白色念珠菌中，只有三对具有相同的RAPD模式。我院麻醉重症监护病房的大多数念珠菌感染似乎是由内源性菌株引起的。白色念珠菌感染的外源性传播较少发生。
