Use of cost effective and rapid molecular tools for identification of species, opportunistic pathogens.

BACKGROUND AND PURPOSE

Candidiasis is a widespread fungal infection caused by different species. Rapid identification of species in clinical laboratory is becoming increasingly important since the identification and discrimination of ethological agents for early treatment. We aimed at molecular identification of commonly species isolated from clinical samples by using both PCR-RFLP assay and amplification of hwp1 gene.

MATERIALS AND METHODS

Clinical samples comprising of vaginal specimens ,cutaneous, sputum, bronchoalveolar lavage(BAL,( and blood cultures were recovered from suspected patients. isolates were initially identified phenotypically and confirmed by molecular approaches based on restriction fragment length polymorphism (PCR-RFLP (with restriction enzyme. Amplification of hwp1 gene was performed for discrimination of from and .

RESULTS

The most abundant species were (n=67; 44.6 %), (n=10; 20 %), (n=20; 13.3 %), (n=12; 8 %), (n=11; 7.3 %). Out of 67 species, 6 species identified as and 4 species identified as .

CONCLUSION

High frequency of non- species and differences in levels of susceptibility to the antifungal agents are important issues in medicine .Therefore, to manage the -related infections properly, molecular diagnostic methods would be fast, reliable and even cost-effective approaches for identification of species.