Marcon Lionel, Melnyk Oleg, Stiévenard Didier
Institut d'Electronique de Microélectronique et de Nanotechnologie, UMR CNRS 8520, Dpt ISEN, 41 Bd Vauban, Lille Cedex, France.
Biosens Bioelectron. 2008 Feb 28;23(7):1185-8. doi: 10.1016/j.bios.2007.10.027. Epub 2007 Nov 9.
We present the electrical detection of immunoglobulin G (IgGs) from human serum using a nanogap-based biosensor. The detection method is based on the capture of IgGs by a probe immobilized between gold nanoelectrodes of 30-90nm spacing. The captured IgGs are further reacted with secondary antibodies labelled with gold nanoparticles (GNPs). Insertion of GNPs into the nanogap resulted in increasing the conductance through the nanogap. The use of a chip with 90 nanogaps enabled the calculation of a quality factor for the detection which, coupled with a non-linear regression analysis of the data, easily discriminated specific and differential capture of human antibodies by arrayed probes. We obtained a 500-fold higher quality factor with protein A compared to goat anti-murine antibodies. This method can be applied, through these proof-of-concept experiments, to the detection of protein-protein interactions in biological samples.
我们展示了一种基于纳米间隙生物传感器对人血清中免疫球蛋白G（IgGs）进行电学检测的方法。该检测方法基于固定在间距为30 - 90nm的金纳米电极之间的探针捕获IgGs。捕获的IgGs再与标记有金纳米颗粒（GNPs）的二抗反应。GNPs插入纳米间隙导致通过纳米间隙的电导增加。使用具有90个纳米间隙的芯片能够计算检测的品质因数，结合数据的非线性回归分析，可轻松区分阵列探针特异性和差异性捕获人抗体的情况。与山羊抗鼠抗体相比，我们使用蛋白A获得了高500倍的品质因数。通过这些概念验证实验，该方法可应用于生物样品中蛋白质 - 蛋白质相互作用的检测。
