Luo Chunxiong, Fu Qiang, Li Hao, Xu Luping, Sun Manhui, Ouyang Qi, Chen Yong, Ji Hang
Center for Microfluidic and Nanotechnology, School of Physics, Peking University, Beijing, 100871, China.
Lab Chip. 2005 Jul;5(7):726-9. doi: 10.1039/b500221d. Epub 2005 Apr 19.
A simple but highly specific immunoassay system for goat anti-human IgG has been developed using gold nanoparticles and microfluidic techniques. The assay is based on the deposition of gold nanoparticles that are coated with protein antigens in the presence of their corresponding antibodies to microfluidic channel surface. The effects of time accumulation, the flow velocity, and the concentration of antibodies to the red light absorption percentage (RAP) of deposition were investigated with an ordinary optical microscope. By controlling the reaction time and flow velocity, a dynamic range of 3 orders of magnitude and a detection sensitivity of 10 ng ml(-1) of goat anti-human IgG were achieved. Because of its simplicity and flexibility, this new technique should be useful for fast, highthroughput screening of antibodies in clinical diagnostic applications.
利用金纳米颗粒和微流控技术开发了一种用于山羊抗人IgG的简单但高度特异的免疫分析系统。该分析基于在相应抗体存在下，包被有蛋白质抗原的金纳米颗粒沉积到微流控通道表面。用普通光学显微镜研究了时间累积、流速和抗体浓度对沉积的红光吸收百分比（RAP）的影响。通过控制反应时间和流速，实现了3个数量级的动态范围和10 ng ml(-1)的山羊抗人IgG检测灵敏度。由于其简单性和灵活性，这项新技术在临床诊断应用中用于抗体的快速、高通量筛选应该是有用的。
