细胞培养中的朊病毒株鉴别：细胞板检测法
Prion strain discrimination in cell culture: the cell panel assay.
作者信息
Mahal Sukhvir P, Baker Christopher A, Demczyk Cheryl A, Smith Emery W, Julius Christian, Weissmann Charles
机构信息
Department of Infectology, Scripps Florida, 5353 Parkside Drive, Jupiter, FL 33458, USA.
出版信息
Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20908-13. doi: 10.1073/pnas.0710054104. Epub 2007 Dec 11.
Prions are thought to consist mainly or entirely of misfolded PrP, a constitutively expressed host protein. Prions associated with the same PrP sequence may occur in the form of different strains; the strain phenotype is believed to be encoded by the conformation of the PrP. Some cell lines can be persistently infected by prions and, interestingly, show preference for certain strains. We report that a cloned murine neuroblastoma cell population, N2a-PK1, is highly heterogeneous in regard to its susceptibility to RML and 22L prions. Remarkably, sibling subclones may show very different relative susceptibilities to the two strains, indicating that the responses can vary independently. We have assembled four cell lines, N2a-PK1, N2a-R33, LD9 and CAD5, which show widely different responses to prion strains RML, 22L, 301C, and Me7, into a panel that allows their discrimination in vitro within 2 weeks, using the standard scrapie cell assay (SSCA).
朊病毒被认为主要或完全由错误折叠的PrP组成，PrP是一种组成型表达的宿主蛋白。与相同PrP序列相关的朊病毒可能以不同毒株的形式出现；毒株表型被认为由PrP的构象编码。一些细胞系可被朊病毒持续感染，有趣的是，它们对某些毒株表现出偏好。我们报道，一个克隆的小鼠神经母细胞瘤细胞群体N2a-PK1，在对RML和22L朊病毒的易感性方面高度异质。值得注意的是，同胞亚克隆对这两种毒株可能表现出非常不同的相对易感性，这表明反应可以独立变化。我们汇集了四个细胞系，N2a-PK1、N2a-R33、LD9和CAD5，它们对朊病毒毒株RML、22L、301C和Me7表现出广泛不同的反应，形成了一个面板，使用标准羊瘙痒病细胞测定法（SSCA），能够在2周内在体外区分它们。
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