Shimizu T, Usuda N, Sugenoya A, Masuda H, Hagiwara M, Hidaka H, Nagata T, Iida F
Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
Cell Mol Biol. 1991;37(8):813-21.
We report immunohistochemical evidence for the overexpression of protein kinase C in various proliferative diseases of human thyroid. Immunohistochemical characterization of various surgically removed thyroid tissues, viz., cancer tissues: papillary carcinoma and follicular carcinoma; adenoma tissues: tubular, trabecular and colloid adenomas; adenomatous goiter; and normal thyroid was done using the monospecific monoclonal antibodies MC-1a, MC-2a and MC-3a, each of which is specific for types I, II and III isozymes of protein kinase C, respectively. For protein kinase C type II, a remarkable difference in staining intensity was noted between the cancerous and normal tissues. The cytoplasm of papillary and follicular carcinoma cells stained more intensely than that of normal thyroid cells. In the benign tumor and adenomatous goiter tissues, stronger staining was noted in the papilliform-proliferating portion and cubic epithelial cells. In the normal thyroid tissues, epithelial cells of greater height were more strongly stained than simple squamous epithelial cells. These results indicated that protein kinase C type II isozyme is expressed in larger amounts in cancerous and proliferative tissues of the human thyroid.
我们报告了蛋白激酶C在人类甲状腺各种增殖性疾病中过表达的免疫组化证据。使用单特异性单克隆抗体MC-1a、MC-2a和MC-3a对各种手术切除的甲状腺组织进行免疫组化特征分析，这些抗体分别对蛋白激酶C的I型、II型和III型同工酶具有特异性。所分析的组织包括癌组织：乳头状癌和滤泡状癌；腺瘤组织：管状、小梁状和胶样腺瘤；腺瘤性甲状腺肿；以及正常甲状腺。对于蛋白激酶C II型，在癌组织和正常组织之间观察到染色强度存在显著差异。乳头状和滤泡状癌细胞的细胞质染色比正常甲状腺细胞更强烈。在良性肿瘤和腺瘤性甲状腺肿组织中，乳头状增殖部分和立方上皮细胞染色更强。在正常甲状腺组织中，较高的上皮细胞比单层扁平上皮细胞染色更强。这些结果表明，蛋白激酶C II型同工酶在人类甲状腺的癌组织和增殖组织中表达量更高。
