Murakami S
Second Department of Surgery, Tokyo Medical and Dental University, Japan.
Nihon Geka Gakkai Zasshi. 1988 Jun;89(6):921-30.
An increase in DNA synthesis runs parallel with an increase in the activity of cytoplasmic thymidine kinase (TK), an enzyme that catalyses the phosphorylation of deoxythymidine via the pyrimidine salvage pathway. In this work, I measured TK and its isozyme activities in normal thyroid tissue, Basedow's disease, adenomatous goiter, adenoma and adenocarcinoma of human thyroid glands. TK activity was assayed by the method of Taylor et al. The average TK activities in Basedow's disease, adenomatous goiter, adenoma and adenocarcinoma were 1.78, 1.75, 2.98 and 3.33 times than that in normal thyroid tissue, respectively. TK isozymes were separated by DEAE cellulose (DE-52, Whatman, Kent, UK) column chromatography (1.5 X 5.0 cm). The activities eluted at NaCl concentrations of 0M, 0.1M and 0. 2 M were named peak A,B and C, respectively. The average activities in peaks A and C were not significantly different from each other in these diseases. But the average activity of peak B in the thyroid adenocarcinoma was significantly higher (2.3 fold) than that in normal thyroid tissue. As the activity of this isozyme was not affected by deoxycytidine triphosphate (dCTP), it may be involved in DNA replication closely.
DNA合成的增加与细胞质胸苷激酶（TK）活性的增加同步，胸苷激酶是一种通过嘧啶补救途径催化脱氧胸苷磷酸化的酶。在这项研究中，我测定了正常甲状腺组织、格雷夫斯病、腺瘤性甲状腺肿、甲状腺腺瘤和腺癌中TK及其同工酶的活性。TK活性采用泰勒等人的方法进行测定。格雷夫斯病、腺瘤性甲状腺肿、腺瘤和腺癌中的TK平均活性分别是正常甲状腺组织的1.78倍、1.75倍、2.98倍和3.33倍。TK同工酶通过DEAE纤维素（DE-52，Whatman，英国肯特）柱色谱法（1.5×5.0厘米）进行分离。在NaCl浓度为0M、0.1M和0.2M时洗脱的活性分别命名为峰A、B和C。在这些疾病中，峰A和C的平均活性彼此之间无显著差异。但甲状腺腺癌中峰B的平均活性显著高于正常甲状腺组织（2.3倍）。由于该同工酶的活性不受三磷酸脱氧胞苷（dCTP）的影响，它可能与DNA复制密切相关。
