[从临床样本中分离出的对甲氧西林敏感和耐药的金黄色葡萄球菌中特定肠毒素和中毒性休克综合征毒素基因的出现情况]

[Occurrence of selected enterotoxins and toxic shock syndrome toxin genes among sensitive and resistant to methicillin Staphylococcus aureus isolated from clinical samples].

作者信息

Garbacz Katarzyna, Dajnowska-Stanczewa Agata, Piechowicz Lidia

机构信息

Zakład Mikrobiologii Lekarskiej Akademii Medycznej w Gdańsku.

出版信息

Med Dosw Mikrobiol. 2007;59(3):201-6.

PMID:18078114

Abstract

The aim of study was to estimate frequency of occurrence of enterotoxins (sea-sed) and TSST-1 (tst) genes. One hundred seven methicillin-sensitive and one hundred three methicillin-resistant strains of S. aureus isolated from hospital patients in 21 medical centers, in majority from the region of Gdansk were examined. The presence of selected toxins genes was detected by multiplex PCR. The results showed that almost 80% of MRSA strains were positive for sea gene, in contrast to MSSA (17,8%). Both MSSA and MRSA strains were rarely positive for the presence of other enterotoxins genes seb, sec, sed (less than 10%) and a tst gene was detected in about 15% of them. No correlation between presence of the particular genes and clinical samples was observed.

摘要

本研究的目的是估计肠毒素（sea-sed）和TSST-1（tst）基因的出现频率。对从21个医疗中心的医院患者中分离出的107株甲氧西林敏感金黄色葡萄球菌和103株甲氧西林耐药金黄色葡萄球菌进行了检测，其中大部分菌株来自格但斯克地区。通过多重PCR检测所选毒素基因的存在情况。结果显示，近80%的耐甲氧西林金黄色葡萄球菌（MRSA）菌株sea基因呈阳性，而甲氧西林敏感金黄色葡萄球菌（MSSA）为17.8%。MSSA和MRSA菌株中其他肠毒素基因seb、sec、sed呈阳性的情况很少（不到10%），约15%的菌株检测到tst基因。未观察到特定基因的存在与临床样本之间的相关性。