Niimi Gen, Hasegawa Kiyoshi, Usuda Nobuteru, Shinzato Masanori, Pereda Jaime, Nagamura Yoichi
Laboratory of Electron Microscopy, Fujita Health University, Toyoake, Aichi, Japan.
Ital J Anat Embryol. 2007 Jul-Sep;112(3):191-8.
Light and electron microscopic examination of first-trimester and term human placental tissues were performed to identify erythrocytes containing hemoglobin in the villous trophoblast cell layer. Erythrocytes were not identified in chorionic villous epithelium at week 7 of gestation. These cells first appeared in the villous cytotrophoblast at week 8, and continued to be present in the villous cytotrophoblast until week 9, as shown by benzidine staining. At week 12 gestation, a cluster of erythrocytes was present in a villous syncytial sprout. At 40 and 41 weeks gestation, erythrocytes were located in the villous cytotrophoblast cell layer. Electron microscopic observations focused on the cytoplasm of villous cytotrophoblast at week 8, the syncytial sprout at week 12 and the cytotrophoblast cell layer at term, confirmed the presence of erythrocytes at an extravascular location, as observed by light microscopy.
对孕早期和足月的人胎盘组织进行光镜和电镜检查，以确定绒毛滋养层细胞层中含有血红蛋白的红细胞。在妊娠第7周的绒毛膜绒毛上皮中未发现红细胞。如联苯胺染色所示，这些细胞在第8周首次出现在绒毛细胞滋养层，并在绒毛细胞滋养层中持续存在至第9周。妊娠第12周时，绒毛合体滋养层芽中有一簇红细胞。在妊娠40周和41周时，红细胞位于绒毛细胞滋养层细胞层。电镜观察聚焦于第8周绒毛细胞滋养层的细胞质、第12周的合体滋养层芽和足月时的细胞滋养层细胞层，证实了光镜下观察到的红细胞存在于血管外位置。
