Rozman D, Komel R
Department of Biochemistry, Medical Faculty, Ljubljana, Slovenia, Yugoslavia.
Curr Genet. 1991 Nov;20(5):385-9. doi: 10.1007/BF00317066.
The filamentous fungus C. lunatus converts progesterone mainly to its 11 beta-hydroxy derivative. C. lunatus transformed with the plasmid pAN 7-1, which contains the E. coli hph gene expressed under the control of the A. nidulans gpd and trpC expression signals, lacks this activity, but exhibits acetyl side chain degradation of progesterone through the reaction scheme progesterone----20 beta-hydroxy-progesterone----delta 4-androstene-3,17-dione---- testolactone + testosterone. The main part of this metabolic pathway is not expressed in the non-transformed strain. It was determined that the site-specific integration of the plasmid into the genome directly influences the expression of genes involved in the bioconversion of steroids.
丝状真菌月形弯孢霉主要将孕酮转化为其11β-羟基衍生物。用质粒pAN 7-1转化的月形弯孢霉缺乏这种活性，该质粒含有在构巢曲霉gpd和trpC表达信号控制下表达的大肠杆菌hph基因，但通过孕酮→20β-羟基孕酮→Δ4-雄烯-3,17-二酮→睾酮内酯+睾酮的反应途径表现出孕酮乙酰侧链降解。这条代谢途径的主要部分在未转化的菌株中不表达。已确定质粒在基因组中的位点特异性整合直接影响参与甾体生物转化的基因的表达。
