Punt P J, Oliver R P, Dingemanse M A, Pouwels P H, van den Hondel C A
TNO Medical Biological Laboratory, Rijswijk, The Netherlands.
Gene. 1987;56(1):117-24. doi: 10.1016/0378-1119(87)90164-8.
A new, heterologous, dominant marker for selection of Aspergillus transformants is described. This marker is based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph). Expression of the hph gene is controlled by A. nidulans gpd and trpC expression signals. An Aspergillus transformation vector was constructed which contains this marker and confers HmB resistance to Aspergillus species. With both A. niger and A. nidulans, transformation frequencies of 5-20 transformants per micrograms vector DNA were obtained. Cotransformation with other vectors was shown to be very efficient in both species, when selection for HmB resistance was applied.
描述了一种用于选择曲霉转化体的新型异源显性标记。该标记基于大肠杆菌潮霉素B（HmB）磷酸转移酶基因（hph）。hph基因的表达由构巢曲霉gpd和trpC表达信号控制。构建了一种曲霉转化载体，其包含该标记并赋予曲霉属对HmB的抗性。对于黑曲霉和构巢曲霉，每微克载体DNA可获得5 - 20个转化体的转化频率。当选择HmB抗性时，与其他载体的共转化在这两个物种中都显示出非常高效。
