MacNeil Joshua A, Doschak Michael R, Zernicke Ronald F, Boyd Steven K
Department of Mechanical and Manufacturing Engineering, Schulich School of Engineering, University of Calgary, 2500 University Drive, NW, Calgary, Alberta, Canada T2N 1N4.
Clin Biomech (Bristol). 2008 Mar;23(3):365-71. doi: 10.1016/j.clinbiomech.2007.10.015. Epub 2007 Dec 19.
Bone changes in experimental post-traumatic osteoarthritis occur early after transection of the anterior cruciate. The purpose of this study was to determine whether antiresorptive bisphosphonate therapy could slow or arrest the periarticular bone architecture and mechanical properties in a post-traumatic model of knee osteoarthritis.
Skeletally mature, female New Zealand white rabbits were assigned to three groups (N=8/group). In two groups, anterior cruciate ligament transection was performed and half were left untreated, and the other half dosed with risedronate (0.01 mg/kg s.c. daily) for a six week period. A third group included age-matched normal controls. At the end of the six week period, all rabbits were euthanized and the femur was scanned by micro-computed tomography to assess morphology and density. Specimen-specific finite element analyses quantified the periarticular bone architecture and mechanical properties.
The untreated transected group had reduced bone volume ratio and mechanical properties compared to the controls (P<0.05) and risedronate-treated transected animals (P<0.02), suggesting bone conservation. Changes in bone volume ratio and mechanical properties of the risedronate-treated transected animals compared to the controls were not detected, indicating that risedronate did not improve these properties relative to the normal controls. The untreated transected group had reduced apparent cancellous bone mineral density and cortical thickness compared to transected animals treated with risedronate (P<0.05).
Bisphosphonate therapy altered the short-term progression of periarticular bone changes including micro-structure and mechanical integrity by slowing early-stage changes to the micro-architecture. These changes following joint trauma may impact the long-term outcome of post-traumatic osteoarthritis.
实验性创伤后骨关节炎的骨骼变化在前交叉韧带横断后早期出现。本研究的目的是确定抗吸收双膦酸盐治疗是否能减缓或阻止创伤后膝关节骨关节炎模型中关节周围骨结构和力学性能的变化。
将骨骼成熟的雌性新西兰白兔分为三组（每组N = 8）。在两组中，进行前交叉韧带横断，其中一半不进行治疗，另一半给予利塞膦酸盐（0.01 mg/kg皮下注射，每日一次），持续六周。第三组包括年龄匹配的正常对照。在六周结束时，对所有兔子实施安乐死，并通过微型计算机断层扫描对股骨进行扫描，以评估形态和密度。特定标本的有限元分析量化了关节周围骨结构和力学性能。
与对照组（P < 0.05）和接受利塞膦酸盐治疗的横断动物组（P < 0.02）相比，未治疗的横断组骨体积比和力学性能降低，提示骨质保存。未检测到接受利塞膦酸盐治疗的横断动物组与对照组相比骨体积比和力学性能的变化，表明相对于正常对照组，利塞膦酸盐并未改善这些性能。与接受利塞膦酸盐治疗的横断动物相比，未治疗的横断组的松质骨表观骨密度和皮质厚度降低（P < 0.05）。
双膦酸盐治疗通过减缓微观结构的早期变化，改变了关节周围骨变化（包括微观结构和力学完整性）的短期进展。关节创伤后的这些变化可能会影响创伤后骨关节炎的长期预后。
