Mariani E, Mangialasche F, Feliziani F T, Cecchetti R, Malavolta M, Bastiani P, Baglioni M, Dedoussis G, Fulop T, Herbein G, Jajte J, Monti D, Rink L, Mocchegiani E, Mecocci P
Institute of Gerontology and Geriatrics, University of Perugia, Italy.
Exp Gerontol. 2008 May;43(5):445-51. doi: 10.1016/j.exger.2007.10.012. Epub 2007 Nov 1.
A large body of experimental research indicates that oxidative stress contributes to the processes related to aging and age-related diseases. Trace elements, particularly zinc (Zn), are essential components of the endogenous enzymatic antioxidant defenses. The aim of this study was to determine the activity of three main antioxidant enzymes in plasma [i.e. superoxide dismutase (pSOD), catalase (CAT), glutathione peroxidase (GPx)] and of SOD in erythrocyte (eSOD) in a group of 1108 healthy elderly subjects from different European countries. The same enzymatic activities were evaluated in a subgroup of 108 subjects before and after Zn supplementation. We observed that eSOD activity increased with age, whereas plasma Zn decreased. Moreover, we found that women showed higher eSOD activity and lower plasma Zn compared to men. There were no age and gender-related differences in the activities of pSOD, CAT and GPx. After Zn supplementation, the activities of Zn-dependent enzymes (pSOD and eSOD), as well as plasma Zn concentration, were significantly higher than before supplementation. These results were not influenced by age, gender, plasma Zn variations (Delta Zn) and geographic area. These data suggest the potential beneficial effects of Zn supplementation on Zn-dependent antioxidant enzymes in healthy elderly subjects.
大量实验研究表明，氧化应激促成了与衰老及老年相关疾病有关的过程。微量元素，尤其是锌（Zn），是内源性酶抗氧化防御的重要组成部分。本研究的目的是测定来自不同欧洲国家的1108名健康老年受试者血浆中三种主要抗氧化酶[即超氧化物歧化酶（pSOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）]的活性以及红细胞中超氧化物歧化酶（eSOD）的活性。在108名受试者补充锌之前和之后对相同的酶活性进行了评估。我们观察到eSOD活性随年龄增加而升高，而血浆锌含量下降。此外，我们发现女性的eSOD活性高于男性，血浆锌含量低于男性。pSOD、CAT和GPx的活性不存在与年龄和性别相关的差异。补充锌之后，锌依赖性酶（pSOD和eSOD）的活性以及血浆锌浓度均显著高于补充之前。这些结果不受年龄、性别、血浆锌变化量（ΔZn）和地理区域的影响。这些数据表明补充锌对健康老年受试者锌依赖性抗氧化酶具有潜在的有益作用。
