Taysi Seyithan, Cikman Oztekin, Kaya Abdullah, Demircan Berna, Gumustekin Kenan, Yilmaz Adnan, Boyuk Abdullah, Keles Mustafa, Akyuz Mehmet, Turkeli Mehmet
Department of Biochemistry, Nenehatun Obstetric and Gynecology Hospital, Erzurum, Turkey.
Biol Trace Elem Res. 2008 Summer;123(1-3):161-7. doi: 10.1007/s12011-008-8095-x. Epub 2008 Feb 14.
The aim of this study was to evaluate the lipid peroxidation, nitric oxide (NO), and free radical scavenging enzyme activities in erythrocytes of zinc (Zn)-deficient rats and to investigate the relationship among these parameters in either group. Sixteen male rats with a weight of 40-50 g were used for the experiment. The rats were divided into control (n = 8) and Zn-deficient groups. At the end of the experiment, the animals were anesthetized with ketamine-HCl (Ketalar, 20 mg/kg(-1), i.p.), and the blood was collected by cardiac puncture after thoracotomy. Blood samples were collected in vacutainer tubes without and with K(3)-EDTA as anticoagulant. Erythrocyte catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GRD), glutathione-S-transferase (GST), superoxide dismutase (SOD) activities, total (enzymatic plus nonenzymatic) superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), antioxidant potential (AOP), and serum zinc (Zn) values in the Zn-deficient group were significantly lower than those of the control group, whereas NO and malondialdehyde (MDA) levels were significantly higher than those of the control group. The results show that Zn deficiency causes a decrease in antioxidant defense system and an increase in oxidative stress in erythrocyte of rats.
本研究旨在评估缺锌大鼠红细胞中的脂质过氧化、一氧化氮（NO）和自由基清除酶活性，并探究两组中这些参数之间的关系。选用16只体重为40 - 50 g的雄性大鼠进行实验。将大鼠分为对照组（n = 8）和缺锌组。实验结束时，用氯胺酮 - HCl（凯他敏，20 mg/kg⁻¹，腹腔注射）麻醉动物，开胸后通过心脏穿刺采集血液。血液样本分别采集于不含和含有K₃ - EDTA作为抗凝剂的真空采血管中。缺锌组红细胞过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH - Px）、谷胱甘肽还原酶（GRD）、谷胱甘肽 - S - 转移酶（GST）、超氧化物歧化酶（SOD）活性、总（酶促加非酶促）超氧化物清除活性（TSSA）、非酶促超氧化物清除活性（NSSA）、抗氧化能力（AOP）以及血清锌（Zn）值均显著低于对照组，而NO和丙二醛（MDA）水平显著高于对照组。结果表明，锌缺乏导致大鼠红细胞抗氧化防御系统下降和氧化应激增加。
