Hoffert Jason D, Knepper Mark A
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA.
Anal Biochem. 2008 Apr 1;375(1):1-10. doi: 10.1016/j.ab.2007.11.023. Epub 2007 Nov 22.
Shotgun phosphoproteomics employs liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) to analyze phosphopeptides from complex protein mixtures, allowing detection and quantification of phosphorylation events on a global scale. Within the past few years, this powerful technique has been used to uncover novel phosphorylation sites as well as explore changes in protein phosphorylation during cellular signaling in various systems. This review presents a general overview of current phosphoproteomic methodologies, including summaries of various approaches to phosphopeptide enrichment, alternative MS fragmentation strategies, and the latest software for analysis of phosphopeptide data sets.
鸟枪法磷酸化蛋白质组学采用液相色谱-串联质谱联用技术（LC-MS/MS）分析复杂蛋白质混合物中的磷酸化肽段，从而能够在全球范围内检测和定量磷酸化事件。在过去几年中，这项强大的技术已被用于发现新的磷酸化位点，并探索各种系统中细胞信号传导过程中蛋白质磷酸化的变化。本综述概述了当前的磷酸化蛋白质组学方法，包括各种磷酸化肽段富集方法、替代质谱裂解策略以及用于分析磷酸化肽段数据集的最新软件的总结。
