Chapman Rob D, Heidemann Martin, Albert Thomas K, Mailhammer Reinhard, Flatley Andrew, Meisterernst Michael, Kremmer Elisabeth, Eick Dirk
Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany.
Science. 2007 Dec 14;318(5857):1780-2. doi: 10.1126/science.1145977.
RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Differential phosphorylation of serine-2 and serine-5 at the 5' and 3' regions of genes appears to coordinate the localization of transcription and RNA processing factors to the elongating polymerase complex. Using monoclonal antibodies, we reveal serine-7 phosphorylation on transcribed genes. This position does not appear to be phosphorylated in CTDs of less than 20 consensus repeats. The position of repeats where serine-7 is substituted influenced the appearance of distinct phosphorylated forms, suggesting functional differences between CTD regions. Our results indicate that restriction of serine-7 epitopes to the Linker-proximal region limits CTD phosphorylation patterns and is a requirement for optimal gene expression.
RNA聚合酶II以其大的羧基末端重复结构域（CTD）为特征，该结构域由共有七肽Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7的重复序列组成。基因5'和3'区域丝氨酸-2和丝氨酸-5的差异磷酸化似乎协调了转录和RNA加工因子在延伸的聚合酶复合物上的定位。我们使用单克隆抗体揭示了转录基因上的丝氨酸-7磷酸化。在少于20个共有重复序列的CTD中，该位点似乎未被磷酸化。丝氨酸-7被取代的重复序列位置影响了不同磷酸化形式的出现，表明CTD区域之间存在功能差异。我们的结果表明，丝氨酸-7表位限制在接头近端区域限制了CTD磷酸化模式，并且是最佳基因表达的必要条件。
