Higgins S E, Higgins J P, Wolfenden A D, Henderson S N, Torres-Rodriguez A, Tellez G, Hargis B
Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
Poult Sci. 2008 Jan;87(1):27-31. doi: 10.3382/ps.2007-00210.
We evaluated the effect of a Lactobacillus-based probiotic culture (FM-B11) for reduction of Salmonella Enteritidis in neonatal broiler chicks. In all experiments, chicks were challenged with approximately 10(4) cfu of Salmonella Enteritidis upon arrival at our laboratory, and the treatments were administered 1 h postchallenge. Cecal tonsil samples were obtained 24 h posttreatment and enriched for Salmonella Enteritidis recovery. The first experiment compared the effects of oral administration of doses of 10(4), 10(6), and 10(8) cfu/chick. In this experiment, doses of 10(6) and 10(8) both significantly reduced Salmonella Enteritidis recovery compared with controls (15 vs. 85% Salmonella Enteritidis positive), but 10(4) cfu did not significantly reduce Salmonella Enteritidis recovery. The second experiment compared the efficacy of oral administration of the live probiotic culture, with or without supernatant removed, to inactivated cultures or supernatant alone. Live probiotic organisms, with or without supernatant, significantly reduced Salmonella Enteritidis in this experiment, but inactivated or cell-free treatments did not reduce Salmonella Enteritidis. In the final 2 experiments, differing doses of probiotic culture were administered on the vent lips, where the treatment was taken into the lower gastrointestinal tract via cloacal drinking. Concentrations of probiotic culture from 10(2) to 10(7) cfu/chick significantly reduced Salmonella Enteritidis, and there was no difference in Salmonella Enteritidis recovery between treatment concentrations. These data suggest that this Lactobacillus-based probiotic culture may be efficacious for reduction of Salmonella Enteritidis in neonatal chicks.
我们评估了一种基于乳酸杆菌的益生菌培养物（FM-B11）对降低新生肉鸡肠炎沙门氏菌感染的效果。在所有实验中，雏鸡抵达我们实验室后即受到约10⁴ cfu肠炎沙门氏菌的攻击，攻击后1小时给予处理。处理后24小时采集盲肠扁桃体样本，进行富集以回收肠炎沙门氏菌。第一个实验比较了每只雏鸡口服10⁴、10⁶和10⁸ cfu剂量的效果。在该实验中，与对照组相比，10⁶和10⁸ cfu剂量均显著降低了肠炎沙门氏菌的回收率（肠炎沙门氏菌阳性率分别为15%和85%），但10⁴ cfu并未显著降低肠炎沙门氏菌的回收率。第二个实验比较了口服有或无去除上清液的活益生菌培养物、灭活培养物或单独上清液的效果。在该实验中，有或无上清液的活益生菌显著降低了肠炎沙门氏菌，但灭活或无细胞处理并未降低肠炎沙门氏菌。在最后两个实验中，在泄殖腔唇上给予不同剂量的益生菌培养物，通过泄殖腔饮水使处理进入下胃肠道。每只雏鸡10²至10⁷ cfu的益生菌培养物浓度均显著降低了肠炎沙门氏菌，处理浓度之间的肠炎沙门氏菌回收率无差异。这些数据表明，这种基于乳酸杆菌的益生菌培养物可能对降低新生雏鸡肠炎沙门氏菌感染有效。
