Wada Ken-Ichi, Taniguchi Akiyoshi, Kobayashi Jun, Yamato Masayuki, Okano Teruo
Cell-Sensing Group, Biomaterials Center, National Institute for Materials Science, 1-1, Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Biotechnol Bioeng. 2008 Apr 15;99(6):1513-7. doi: 10.1002/bit.21718.
We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents. The fluorescence in the sensor cells increased in a CdCl(2) dose-dependent manner in the microfluidic channels. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high-throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (micro-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.
我们在此报告一种基于活细胞的传感器芯片，它是在微流体通道中制造的，在该通道中实现了多个层流。此外，我们建立了一种细胞毒性传感器细胞系，该细胞系用源自热休克蛋白70B'（HSP70B'）启动子的绿色荧光蛋白质（GFP）质粒转染，该启动子由细胞毒性试剂诱导。在微流体通道中，传感器细胞中的荧光以CdCl₂剂量依赖性方式增加。在该系统中，可以快速、灵敏和高通量地定量检测细胞毒性试剂。传感器细胞与微流体系统的结合将为微全分析系统（micro-TAS）技术的发展提供重要基础，并可应用于毒理学、环境评估和药物筛选。
