Cell-Materials Interaction Group, Biomaterials Unit, Nano-Bio Field, National Institute for Materials Science, 1-1, Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Sensors (Basel). 2011;11(7):7219-30. doi: 10.3390/s110707219. Epub 2011 Jul 18.
The increasing use of nanomaterials in consumer and industrial products has aroused concerns regarding their fate in biological systems. An effective detection method to evaluate the safety of bio-nanomaterials is therefore very important. Titanium dioxide (TiO(2)), which is manufactured worldwide in large quantities for use in a wide range of applications, including pigment and cosmetic manufacturing, was once thought to be an inert material, but recently, more and more studies have indicated that TiO(2) nanoparticles (TiO(2) NPs) can cause inflammation and be harmful to humans by causing lung and brain problems. In order to evaluate the safety of TiO(2) NPs for the environment and for humans, sensor cells for inflammation detection were developed, and these were transfected with the Toll-like receptor 4 (TLR4) gene and Nuclear Factor Kappa B (NF-κB) reporter gene. NF-κB as a primary cause of inflammation has received a lot of attention, and it can be activated by a wide variety of external stimuli. Our data show that TiO(2) NPs-induced inflammation can be detected by our sensor cells through NF-κB pathway activation. This may lead to our sensor cells being used for bio-nanomaterial safety evaluation.
纳米材料在消费和工业产品中的应用日益广泛，这引起了人们对其在生物系统中命运的关注。因此，一种有效的检测方法来评估生物纳米材料的安全性非常重要。二氧化钛（TiO(2)），全球范围内大量生产用于各种应用，包括颜料和化妆品制造，曾经被认为是一种惰性材料，但最近越来越多的研究表明，TiO(2)纳米颗粒（TiO(2) NPs）可通过引起肺部和大脑问题引起炎症并对人体造成危害。为了评估 TiO(2) NPs 对环境和人类的安全性，开发了用于炎症检测的传感器细胞，并将 Toll 样受体 4 (TLR4) 基因和核因子 Kappa B (NF-κB) 报告基因转染到这些细胞中。NF-κB 作为炎症的主要原因受到了广泛关注，它可以被多种外部刺激激活。我们的数据表明，我们的传感器细胞可以通过 NF-κB 通路的激活来检测 TiO(2) NPs 诱导的炎症。这可能导致我们的传感器细胞被用于生物纳米材料的安全性评估。
