在细胞生物反应器中固定化黄孢原毛平革菌BKM-F-1767生产锰过氧化物酶

Manganese peroxidase production by immobilized Phanerochaete chrysosporium BKM-F-1767 in a cell bioreactor.

作者信息

Urek Raziye Ozturk, Pazarlioglu Nurdan Kasikara

机构信息

Faculty of Education, Department of Chemistry, Dokuz Eylul University, Buca, Izmir, Turkey.

出版信息

Prep Biochem Biotechnol. 2008;38(1):1-12. doi: 10.1080/10826060701774296.

DOI:10.1080/10826060701774296

PMID:18080906

Abstract

Manganese-dependent peroxidase (MnP) production was performed in an immobilized cell bioreactor in which Phanerochaete chrysosporium BKM-F-1767 was immobilized on polystyrene foam. The immobilized cell culture yielded significantly greater MnP activity than the conventional stationary liquid culture. Cultivation was carried out in batch mode; the effect of glucose concentration was investigated and growth kinetics parameters were found as, micromax=0.59 day(-1), Ks=0.33 g/L and Kss=14.5. Batch operation led to maximum MnP (770.82 U/L) in the culture medium containing 0.05% Tween 80, 10 g/L glucose, and 174 microM Mn2+ at 37 degrees C and pH 4.5. Enzyme productivity was obtained as 110.12 U/day/L.

摘要

在一个固定化细胞生物反应器中进行了锰依赖过氧化物酶（MnP）的生产，该反应器将黄孢原毛平革菌BKM-F-1767固定在聚苯乙烯泡沫上。固定化细胞培养产生的MnP活性明显高于传统的静止液体培养。培养以分批模式进行；研究了葡萄糖浓度的影响，并发现生长动力学参数为：最大比生长速率（micromax）=0.59天⁻¹，底物饱和常数（Ks）=0.33 g/L，产物抑制常数（Kss）=14.5。在含有0.05%吐温80、10 g/L葡萄糖和174 μM Mn²⁺的培养基中，于37℃和pH 4.5条件下进行分批操作，培养基中MnP的产量达到最大值（770.82 U/L）。酶的生产效率为110.12 U/天/L。