Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, P.R. China.
Water Sci Technol. 2010;62(4):947-55. doi: 10.2166/wst.2010.320.
The performance of a fluidized bed reactor using immobilized Phanerochaete chrysosporium to remove 2,4-dichlorophenol (2,4-DCP) from aqueous solution was investigated. The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4-DCP degradation was examined. Results showed that Lip and Mnp were not essential to 2,4-DCP degradation while their presence enhanced the degradation process and reaction rate. In sequential batch experiment, the bioactivity of immobilized cells was recovered and improved during the culture and the maximum degradation rate constant of 13.95 mg (Ld)⁻¹ could be reached. In continuous bioreactor test, the kinetic behavior of the Phanerochaete chrysosporium immobilized on loofa sponge was found to follow the Monod equation. The maximum reaction rate was 7.002 mg (Lh)⁻¹, and the saturation constant was 26.045 mg L⁻¹.
使用固定化白腐菌去除水溶液中 2,4-二氯苯酚（2,4-DCP）的流化床反应器的性能进行了研究。考察了白腐菌分泌的木质素过氧化物酶（LiP）和锰过氧化物酶（MnP）对 2,4-DCP 降解的贡献。结果表明，LiP 和 MnP 对 2,4-DCP 的降解不是必需的，但其存在会促进降解过程和反应速率。在连续批实验中，固定化细胞的生物活性在培养过程中得到恢复和提高，最大降解速率常数可达 13.95mg（Ld）⁻¹。在连续生物反应器试验中，发现固定在丝瓜络上的白腐菌的动力学行为符合 Monod 方程。最大反应速率为 7.002mg（Lh）⁻¹，饱和常数为 26.045mg L⁻¹。
