Cayla J-L, Maire P, Duvallet A, Wahrmann J P
Génétique et développement du système neuromusculaire, Cochin, Université de Paris V, Paris, France.
Int J Sports Med. 2008 Jun;29(6):460-5. doi: 10.1055/s-2007-965359. Epub 2007 Dec 17.
Sedentary and trained rat groups were studied. Each of these groups was either erythropoietin or placebo treated. Erythropoietin treatment increased significantly all haematological parameters studied. Training per se failed to modify haematological parameters. In a second time, we studied the specific activity of several oxidative enzymes in three different muscles. In sedentary rats, erythropoietin treatment increased significantly the specific activities of cytochrome c oxidase and L-3-hydroxyacyl CoA dehydrogenase in the soleus and those of L-3-hydroxyacyl CoA dehydrogenase and phosphofructokinase in both locomotor muscles. Training increased the oxidative enzymes activities in all muscles studied. In trained rats, effects of erythropoietin and training on oxidative enzymes activities were additive. In all erythropoietin treated muscles, the expression of slow twitch myosin light chains and oxidative myosin heavy chains increased. A similar phenomenon took place in all trained groups for light chains and in placebo treated trained rats for heavy chains. In trained groups, the effects of the hormone and of training were additive. Our results suggest strongly that two different mechanisms are involved in the response of skeletal muscles to erythropoietin treatment and to endurance training and probably whole body endurance is affected by erythropoietin treatment by an increase of oxygenation of all tissues.
对久坐不动组和训练组大鼠进行了研究。每组大鼠均接受促红细胞生成素或安慰剂治疗。促红细胞生成素治疗显著提高了所有研究的血液学参数。训练本身未能改变血液学参数。其次，我们研究了三种不同肌肉中几种氧化酶的比活性。在久坐不动的大鼠中，促红细胞生成素治疗显著提高了比目鱼肌中细胞色素c氧化酶和L-3-羟酰基辅酶A脱氢酶的比活性，以及运动肌肉中L-3-羟酰基辅酶A脱氢酶和磷酸果糖激酶的比活性。训练增加了所有研究肌肉中的氧化酶活性。在训练有素的大鼠中，促红细胞生成素和训练对氧化酶活性的影响是相加的。在所有接受促红细胞生成素治疗的肌肉中，慢肌球蛋白轻链和氧化型肌球蛋白重链的表达增加。在所有训练组中，轻链出现了类似现象，在接受安慰剂治疗的训练有素的大鼠中，重链也出现了类似现象。在训练组中，激素和训练的影响是相加的。我们的结果强烈表明，骨骼肌对促红细胞生成素治疗和耐力训练的反应涉及两种不同的机制，并且促红细胞生成素治疗可能通过增加所有组织的氧合作用来影响全身耐力。
