Dalley Deanna, Davé Dipesh, Lesellier Sandrine, Palmer Simonette, Crawshaw Timothy, Hewinson R Glyn, Chambers Mark
TB Research Group, Department of Statutory and Exotic Bacteria, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK.
Tuberculosis (Edinb). 2008 May;88(3):235-43. doi: 10.1016/j.tube.2007.11.001. Epub 2007 Dec 20.
In this paper we report the development of a sensitive and specific assay for the detection of tuberculosis (TB) in European badgers (Meles meles), based on the stimulation of lymphocytes in whole-blood culture and the subsequent detection of gamma-interferon (IFNgamma) by sandwich ELISA. The comparative levels of IFNgamma produced to bovine and avian tuberculin (B-A) was used as the basis of determining the TB status of badgers, resulting in a more sensitive test than that based on the defined Mycobacterium bovis antigens ESAT6 and CFP10. The assay was evaluated using 235 badgers. The IFNgamma EIA (enzyme immunoassay) based on a monoclonal pair (mEIA) was more sensitive than one using a rabbit polyclonal antiserum (pEIA). At a specificity of 93.6%, the mEIA was 80.9% sensitive, compared to a sensitivity of 74.5% for the pEIA. At the same specificity as the EIA, the current serological ELISA test for TB in badgers (Brock test) had a sensitivity of 48.9%. Only one of the culture positive badgers missed by the mEIA was correctly diagnosed by the Brock test, suggesting that the combination of both a T-cell and serological test has little diagnostic advantage.
在本文中，我们报告了一种用于检测欧洲獾结核病（TB）的灵敏且特异的检测方法的开发，该方法基于全血培养中淋巴细胞的刺激以及随后通过夹心酶联免疫吸附测定（ELISA）检测γ-干扰素（IFNγ）。以对牛型和禽型结核菌素（B - A）产生的IFNγ的比较水平作为确定獾结核病状态的基础，从而得到一种比基于明确的牛分枝杆菌抗原ESAT6和CFP10的检测方法更灵敏的检测方法。使用235只獾对该检测方法进行了评估。基于单克隆抗体对的IFNγ酶免疫测定（EIA）（mEIA）比使用兔多克隆抗血清的测定（pEIA）更灵敏。在特异性为93.6%时，mEIA的灵敏度为80.9%，而pEIA的灵敏度为74.5%。在与EIA相同的特异性下，目前用于检测獾结核病的血清学ELISA检测（布罗克检测）的灵敏度为48.9%。mEIA漏检的培养阳性獾中，只有一只被布罗克检测正确诊断，这表明T细胞检测和血清学检测相结合几乎没有诊断优势。
