Suppr超能文献

单核细胞增生李斯特菌激活驻留腹膜巨噬细胞中的胞质磷脂酶A2α涉及李斯特菌溶血素O和Toll样受体2。

Activation of cytosolic phospholipase A2alpha in resident peritoneal macrophages by Listeria monocytogenes involves listeriolysin O and TLR2.

作者信息

Noor Shahid, Goldfine Howard, Tucker Dawn E, Suram Saritha, Lenz Laurel L, Akira Shizuo, Uematsu Satoshi, Girotti Milena, Bonventre Joseph V, Breuel Kevin, Williams David L, Leslie Christina C

机构信息

Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA.

出版信息

J Biol Chem. 2008 Feb 22;283(8):4744-55. doi: 10.1074/jbc.M709956200. Epub 2007 Dec 14.

Abstract

Eicosanoid production by macrophages is an early response to microbial infection that promotes acute inflammation. The intracellular pathogen Listeria monocytogenes stimulates arachidonic acid release and eicosanoid production from resident mouse peritoneal macrophages through activation of group IVA cytosolic phospholipase A2 (cPLA2alpha). The ability of wild type L. monocytogenes (WTLM) to stimulate arachidonic acid release is partially dependent on the virulence factor listeriolysin O; however, WTLM and L. monocytogenes lacking listeriolysin O (DeltahlyLM) induce similar levels of cyclooxygenase 2. Arachidonic acid release requires activation of MAPKs by WTLM and DeltahlyLM. The attenuated release of arachidonic acid that is observed in TLR2-/- and MyD88-/- macrophages infected with WTLM and DeltahlyLM correlates with diminished MAPK activation. WTLM but not DeltahlyLM increases intracellular calcium, which is implicated in regulation of cPLA2alpha. Prostaglandin E2, prostaglandin I2, and leukotriene C4 are produced by cPLA2alpha+/+ but not cPLA2alpha-/- macrophages in response to WTLM and DeltahlyLM. Tumor necrosis factor (TNF)-alpha production is significantly lower in cPLA2alpha+/+ than in cPLA2alpha-/- macrophages infected with WTLM and DeltahlyLM. Treatment of infected cPLA2alpha+/+ macrophages with the cyclooxygenase inhibitor indomethacin increases TNFalpha production to the level produced by cPLA2alpha-/- macrophages implicating prostaglandins in TNFalpha down-regulation. Therefore activation of cPLA2alpha in macrophages may impact immune responses to L. monocytogenes.

摘要

巨噬细胞产生类花生酸是对微生物感染的早期反应,可促进急性炎症。细胞内病原体单核细胞增生李斯特菌通过激活IVA组胞质磷脂酶A2(cPLA2α),刺激驻留小鼠腹膜巨噬细胞释放花生四烯酸并产生类花生酸。野生型单核细胞增生李斯特菌(WTLM)刺激花生四烯酸释放的能力部分依赖于毒力因子溶血素O;然而,WTLM和缺乏溶血素O的单核细胞增生李斯特菌(DeltahlyLM)诱导的环氧化酶2水平相似。WTLM和DeltahlyLM刺激花生四烯酸释放需要激活丝裂原活化蛋白激酶(MAPK)。在感染WTLM和DeltahlyLM的TLR2-/-和MyD88-/-巨噬细胞中观察到的花生四烯酸释放减弱与MAPK激活减少相关。WTLM而非DeltahlyLM可增加细胞内钙,这与cPLA2α的调节有关。前列腺素E2、前列腺素I2和白三烯C4由cPLA2α+/+而非cPLA2α-/-巨噬细胞响应WTLM和DeltahlyLM产生。在感染WTLM和DeltahlyLM的cPLA2α+/+巨噬细胞中,肿瘤坏死因子(TNF)-α的产生明显低于cPLA2α-/-巨噬细胞。用环氧化酶抑制剂吲哚美辛处理感染的cPLA2α+/+巨噬细胞可使TNFα产生增加至cPLA2α-/-巨噬细胞产生的水平,这表明前列腺素参与TNFα的下调。因此,巨噬细胞中cPLA2α的激活可能影响对单核细胞增生李斯特菌的免疫反应。

相似文献

3
Low molecular weight hyaluronan activates cytosolic phospholipase A2α and eicosanoid production in monocytes and macrophages.
J Biol Chem. 2014 Feb 14;289(7):4470-88. doi: 10.1074/jbc.M113.515106. Epub 2013 Dec 23.
4
Pathways regulating cytosolic phospholipase A2 activation and eicosanoid production in macrophages by Candida albicans.
J Biol Chem. 2010 Oct 1;285(40):30676-85. doi: 10.1074/jbc.M110.143800. Epub 2010 Jul 18.
8
Cytosolic phospholipase A2α enhances mouse mortality induced by Pseudomonas aeruginosa pulmonary infection via interleukin 6.
Biochimie. 2014 Dec;107 Pt A:95-104. doi: 10.1016/j.biochi.2014.08.018. Epub 2014 Sep 6.
9
Membrane damage during Listeria monocytogenes infection triggers a caspase-7 dependent cytoprotective response.
PLoS Pathog. 2012;8(7):e1002628. doi: 10.1371/journal.ppat.1002628. Epub 2012 Jul 12.
10
Cytosolic phospholipase A2 modulates TLR2 signaling in synoviocytes.
PLoS One. 2015 Apr 20;10(4):e0119088. doi: 10.1371/journal.pone.0119088. eCollection 2015.

引用本文的文献

1
Gram-negative bacteria-driven increase of cytosolic phospholipase A2 leads to activation of Kupffer cells.
Cell Mol Life Sci. 2024 Dec 27;82(1):22. doi: 10.1007/s00018-024-05451-5.
2
Inhibiting lipid droplet biogenesis enhances host protection against hypervirulent Klebsiella pneumoniae infections.
Med Microbiol Immunol. 2024 Nov 14;213(1):26. doi: 10.1007/s00430-024-00807-x.
3
Bacterial cholesterol-dependent cytolysins and their interaction with the human immune response.
Curr Opin Infect Dis. 2024 Jun 1;37(3):164-169. doi: 10.1097/QCO.0000000000001010. Epub 2024 Mar 21.
4
Bacterial-Mediated Tumor Therapy: Old Treatment in a New Context.
Adv Sci (Weinh). 2023 Apr;10(12):e2205641. doi: 10.1002/advs.202205641. Epub 2023 Mar 12.
5
A protein-lipid complex that detoxifies free fatty acids.
Bioessays. 2023 Mar;45(3):e2200210. doi: 10.1002/bies.202200210. Epub 2022 Dec 30.
6
Phagocytes produce prostaglandin E2 in response to cytosolic Listeria monocytogenes.
PLoS Pathog. 2021 Sep 23;17(9):e1009493. doi: 10.1371/journal.ppat.1009493. eCollection 2021 Sep.
7
The Involvement of Lactosylceramide in Central Nervous System Inflammation Related to Neurodegenerative Disease.
Front Aging Neurosci. 2021 Jul 19;13:691230. doi: 10.3389/fnagi.2021.691230. eCollection 2021.
8
cancer vaccines: bridging innate and adaptive immunity.
Curr Clin Microbiol Rep. 2019 Dec;6(4):213-224. doi: 10.1007/s40588-019-00133-4. Epub 2019 Nov 20.

本文引用的文献

1
Sterol and pH interdependence in the binding, oligomerization, and pore formation of Listeriolysin O.
Biochemistry. 2007 Apr 10;46(14):4425-37. doi: 10.1021/bi602497g. Epub 2007 Mar 15.
4
The role of the activated macrophage in clearing Listeria monocytogenes infection.
Front Biosci. 2007 Jan 1;12:2683-92. doi: 10.2741/2364.
5
Properties of the Group IV phospholipase A2 family.
Prog Lipid Res. 2006 Nov;45(6):487-510. doi: 10.1016/j.plipres.2006.05.003. Epub 2006 Jun 15.
7
Listeriolysin O: a key protein of Listeria monocytogenes with multiple functions.
FEMS Microbiol Rev. 2006 Jul;30(4):514-29. doi: 10.1111/j.1574-6976.2006.00021.x.
8
Listeria monocytogenes: a multifaceted model.
Nat Rev Microbiol. 2006 Jun;4(6):423-34. doi: 10.1038/nrmicro1413.
9
A global analysis of cross-talk in a mammalian cellular signalling network.
Nat Cell Biol. 2006 Jun;8(6):571-80. doi: 10.1038/ncb1418. Epub 2006 May 14.
10
Regulation of cytosolic phospholipase A2 activation and cyclooxygenase 2 expression in macrophages by the beta-glucan receptor.
J Biol Chem. 2006 Mar 3;281(9):5506-14. doi: 10.1074/jbc.M509824200. Epub 2006 Jan 3.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验