嗜热栖热放线菌纤维二糖水解酶9A的CBM3b结构域的结晶及初步衍射研究
Crystallization and preliminary diffraction studies of CBM3b of cellobiohydrolase 9A from Clostridium thermocellum.
作者信息
Jindou Sadanari, Petkun Svetlana, Shimon Linda, Bayer Edward A, Lamed Raphael, Frolow Felix
机构信息
Department of Molecular Microbiology and Biotechnology, Tel Aviv University 69978, Israel.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt 12):1044-7. doi: 10.1107/S1744309107054644. Epub 2007 Nov 21.
Abstract
Family 3 carbohydrate-binding modules (CBM3s) are associated with the scaffoldin subunit of the multi-enzyme cellulosome complex and with the family 9 glycoside hydrolases, which are multimodular enzymes that act on plant cell-wall polysaccharides, notably cellulose. Here, the crystallization of CBM3b from cellobiohydrolase 9A is reported. The crystals are tetragonal and belong to space group P4(1) or P4(3). X-ray diffraction data for CBM3b have been collected to 2.68 A resolution on beamline ID14-4 at the ESRF.
摘要
第3家族碳水化合物结合模块（CBM3s）与多酶纤维小体复合物的支架蛋白亚基以及第9家族糖苷水解酶相关，这些多模块酶作用于植物细胞壁多糖，尤其是纤维素。本文报道了来自纤维二糖水解酶9A的CBM3b的晶体结构。晶体为四方晶系，属于空间群P4(1)或P4(3)。在欧洲同步辐射装置的ID14-4光束线上收集到了分辨率为2.68 Å的CBM3b的X射线衍射数据。
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CelI, a noncellulosomal family 9 enzyme from Clostridium thermocellum, is a processive endoglucanase that degrades crystalline cellulose.CelI是一种来自嗜热栖热放线菌的非纤维小体家族9酶，是一种可连续作用的内切葡聚糖酶，可降解结晶纤维素。
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