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Domain coupling in a multimodular cellobiohydrolase CbhA from Clostridium thermocellum.嗜热栖热放线菌多模块纤维二糖水解酶CbhA中的结构域偶联
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Novel architecture of family-9 glycoside hydrolases identified in cellulosomal enzymes of Acetivibrio cellulolyticus and Clostridium thermocellum.在解纤维醋弧菌和嗜热栖热菌的纤维小体酶中鉴定出的9家族糖苷水解酶的新型结构。
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本文引用的文献

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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Novel architecture of family-9 glycoside hydrolases identified in cellulosomal enzymes of Acetivibrio cellulolyticus and Clostridium thermocellum.在解纤维醋弧菌和嗜热栖热菌的纤维小体酶中鉴定出的9家族糖苷水解酶的新型结构。
FEMS Microbiol Lett. 2006 Jan;254(2):308-16. doi: 10.1111/j.1574-6968.2005.00040.x.
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Likelihood-enhanced fast translation functions.似然增强快速翻译功能。
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Interactions between immunoglobulin-like and catalytic modules in Clostridium thermocellum cellulosomal cellobiohydrolase CbhA.嗜热栖热放线菌纤维小体纤维二糖水解酶CbhA中免疫球蛋白样模块与催化模块之间的相互作用
Protein Eng Des Sel. 2004 Nov;17(11):759-69. doi: 10.1093/protein/gzh094. Epub 2004 Dec 13.
5
The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides.纤维小体：用于降解植物细胞壁多糖的多酶机器
Annu Rev Microbiol. 2004;58:521-54. doi: 10.1146/annurev.micro.57.030502.091022.
6
Two new cellulosome components encoded downstream of celI in the genome of Clostridium thermocellum: the non-processive endoglucanase CelN and the possibly structural protein CseP.热纤梭菌基因组中celI下游编码的两种新的纤维小体组分：非连续内切葡聚糖酶CelN和可能的结构蛋白CseP。
Microbiology (Reading). 2003 Feb;149(Pt 2):515-524. doi: 10.1099/mic.0.25959-0.
7
CelI, a noncellulosomal family 9 enzyme from Clostridium thermocellum, is a processive endoglucanase that degrades crystalline cellulose.CelI是一种来自嗜热栖热放线菌的非纤维小体家族9酶，是一种可连续作用的内切葡聚糖酶，可降解结晶纤维素。
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8
Structure of a family IIIa scaffoldin CBD from the cellulosome of Clostridium cellulolyticum at 2.2 A resolution.解纤维素梭菌纤维小体中IIIa家族支架蛋白CBD在2.2埃分辨率下的结构
Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1560-8. doi: 10.1107/s0907444900012889.
9
Cellulosomes-structure and ultrastructure.纤维小体——结构与超微结构
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10
Roles of the catalytic domain and two cellulose binding domains of Thermomonospora fusca E4 in cellulose hydrolysis.嗜热栖热放线菌E4的催化结构域和两个纤维素结合结构域在纤维素水解中的作用。
J Bacteriol. 1998 Apr;180(7):1709-14. doi: 10.1128/JB.180.7.1709-1714.1998.

嗜热栖热放线菌纤维二糖水解酶9A的CBM3b结构域的结晶及初步衍射研究

Crystallization and preliminary diffraction studies of CBM3b of cellobiohydrolase 9A from Clostridium thermocellum.

作者信息

Jindou Sadanari, Petkun Svetlana, Shimon Linda, Bayer Edward A, Lamed Raphael, Frolow Felix

机构信息

Department of Molecular Microbiology and Biotechnology, Tel Aviv University 69978, Israel.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt 12):1044-7. doi: 10.1107/S1744309107054644. Epub 2007 Nov 21.

DOI:10.1107/S1744309107054644
PMID:18084089
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2344089/
Abstract

Family 3 carbohydrate-binding modules (CBM3s) are associated with the scaffoldin subunit of the multi-enzyme cellulosome complex and with the family 9 glycoside hydrolases, which are multimodular enzymes that act on plant cell-wall polysaccharides, notably cellulose. Here, the crystallization of CBM3b from cellobiohydrolase 9A is reported. The crystals are tetragonal and belong to space group P4(1) or P4(3). X-ray diffraction data for CBM3b have been collected to 2.68 A resolution on beamline ID14-4 at the ESRF.

摘要

第3家族碳水化合物结合模块（CBM3s）与多酶纤维小体复合物的支架蛋白亚基以及第9家族糖苷水解酶相关，这些多模块酶作用于植物细胞壁多糖，尤其是纤维素。本文报道了来自纤维二糖水解酶9A的CBM3b的晶体结构。晶体为四方晶系，属于空间群P4(1)或P4(3)。在欧洲同步辐射装置的ID14-4光束线上收集到了分辨率为2.68 Å的CBM3b的X射线衍射数据。