Kislitsyn Yury A, Samygina Valeriya R, Dvortsov Igor A, Lunina Nataliya A, Kuranova Inna P, Velikodvorskaya Galina A
A. V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninsky Prospect 59, Moscow 117333, Russian Federation.
Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Square 2, Moscow 123182, Russian Federation.
Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):217-20. doi: 10.1107/S2053230X15000539. Epub 2015 Jan 28.
The crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding module (CBM) from laminarinase Lic16A of the hyperthermophilic anaerobic bacterium Clostridium thermocellum (ctCBM54) are reported. Recombinant ctCBM54 was prepared using an Escherichia coli/pQE30 overexpression system and was crystallized by the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 2.1 Å resolution using synchrotron radiation. The crystals belonged to space group P6322, with unit-cell parameters a = b = 130.15, c = 131.05 Å. The three-dimensional structure of ctCBM54 will provide valuable information about the structure-function relation of the laminarinase Lic16A and will allow the exploitation of this binding module in biotechnological applications.
报道了嗜热厌氧菌热纤梭菌（Clostridium thermocellum）的海带多糖酶Lic16A的碳水化合物结合模块（CBM，即ctCBM54）的结晶及初步X射线衍射分析。使用大肠杆菌/pQE30过表达系统制备重组ctCBM54，并通过悬滴气相扩散法进行结晶。利用同步辐射收集了分辨率为2.1 Å的X射线衍射数据。晶体属于P6322空间群，晶胞参数a = b = 130.15，c = 131.05 Å。ctCBM54的三维结构将为海带多糖酶Lic16A的结构-功能关系提供有价值的信息，并有助于在生物技术应用中开发这种结合模块。
