Krasnova M A, Makarova M V, Dorozhkova i R, Skotnikova o I, Moroz A M
Probl Tuberk Bolezn Legk. 2007(11):29-32.
The genus Mycobacterium currently comprises more than 90 species of Mycobacterium, of which a third is able to induce human diseases. With a rise in the incidence of diseases induced by non-tuberculosis mycobacteria, tuberculosis caused by M. bovis that is characterized by a severe cause and a high frequency of poor outcomes cannot be remembered. The species of mycobacteria should be identified to establish a diagnosis and to prescribe adequate chemotherapy. For this purpose, cultural, biochemical, chromatographic, and molecular genetic studies are conducted. The present study using the hsp65 gene restriction fragment length polymorphism test on museum mycobacterial strains and strains isolated from the diagnostic material of patients with suspected tuberculosis by means of Hind61 restrictase has provided a clear differentiation of the restriction profiles of MAIS complex mycobacteria and some other species of non-tuberculosis mycobacteria. To determine the species of representatives of M. tuberculosis complex (M. tuberculosis, M. bovis, BCG M. bovis), the authors have successfully used the test system "TUB-dif" developed at the Institute of Molecular Genetics, Russian Academy of Sciences, by applying the chain polymerase reaction of the senX3-regX3 region.
分枝杆菌属目前包含90多种分枝杆菌，其中三分之一能够引发人类疾病。随着非结核分枝杆菌所致疾病发病率的上升，由牛分枝杆菌引起的结核病，其病因严重且不良结局发生率高，已被人们遗忘。应鉴定分枝杆菌的种类以进行诊断并开具适当的化疗药物。为此，要进行培养、生化、色谱和分子遗传学研究。本研究通过Hind61限制性内切酶对博物馆分枝杆菌菌株以及从疑似结核病患者诊断材料中分离出的菌株进行热休克蛋白65（hsp65）基因限制性片段长度多态性检测，已明确区分了MAIS复合群分枝杆菌和其他一些非结核分枝杆菌的限制性图谱。为确定结核分枝杆菌复合群（结核分枝杆菌、牛分枝杆菌、卡介苗牛分枝杆菌）代表菌株的种类，作者成功使用了俄罗斯科学院分子遗传学研究所开发的“TUB-dif”检测系统，通过应用senX3-regX3区域的链聚合酶反应来实现。
