肌成纤维细胞在人正常精囊基质中的分布及作用

Distribution and role of myofibroblasts in human normal seminal vesicle stroma.

作者信息

Shimasaki Nobuyuki, Kuroda Naoto, Miyazaki Eriko, Hayashi Yoshihiro, Toi Makoto, Hiroi Makoto, Enzan Hideaki, Shuin Taro

机构信息

Department of Urology, Program of Tumor Biology and Regulation, Kochi Medical School, Kochi University, Kohasu, Oko-Cho, Nangoku, Kochi, Japan.

出版信息

Med Mol Morphol. 2007 Dec;40(4):208-11. doi: 10.1007/s00795-007-0378-3. Epub 2007 Dec 21.

DOI:10.1007/s00795-007-0378-3

PMID:18085380

Abstract

We studied the distribution of myofibroblasts in the stroma of normal seminal vesicles. Twelve normal seminal vesicles obtained by surgery on the diagnosis of some diseases were selected, and we evaluated the distribution of myofibroblasts in the seminal vesicles using immunohistochemical and electron and immunoelectron microscopic techniques. Immunohistochemically, myofibroblasts, which were positive for alpha-smooth muscle actin (ASMA) and negative for high molecular weight caldesmon (h-CD), were observed in the stroma just beneath the epithelium of normal seminal vesicles. Moreover, an electron microscope examination revealed the presence of spindle or stellate cells in the stroma of the lamina propria beneath the seminal vesicle epithelium, and an immunoelectron microscopic examination showed that these cells were positive for ASMA. Finally, myofibroblasts are distributed in the lamina propria of human normal seminal vesicles and may play an important role in the ejection of sperm plasm.

摘要

我们研究了正常精囊基质中肌成纤维细胞的分布情况。选取了12个因某些疾病诊断而经手术获取的正常精囊，并运用免疫组织化学、电子显微镜及免疫电子显微镜技术评估精囊中肌成纤维细胞的分布。免疫组织化学检测显示，在正常精囊上皮下方的基质中观察到肌成纤维细胞，其α-平滑肌肌动蛋白（ASMA）呈阳性，高分子量钙调蛋白（h-CD）呈阴性。此外，电子显微镜检查发现精囊上皮下方固有层基质中存在梭形或星状细胞，免疫电子显微镜检查表明这些细胞ASMA呈阳性。最后，肌成纤维细胞分布于人类正常精囊的固有层，可能在精子浆射出过程中发挥重要作用。