Park Eungsik, Suh Hyunsuk, Kim Changsoo, Park Seungwoo, Dorsett Dale, Yim Jeongbin
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
IUBMB Life. 2007 Dec;59(12):781-90. doi: 10.1080/15216540701708318.
A P element enhancer trap screen was conducted to identify genes involved in dorsal-ventral boundary formation in Drosophila. The son of Notch (son) gene was identified by the son(2205) enhancer trap insertion, which is a partial loss-of-function mutation. Based on son(2205) mutant phenotypes and genetic interactions with Notch and wingless mutations, we conclude that son participates in wing development, and functions in the Notch signaling pathway at the dorsal-ventral boundary in the wing. Notch signaling pathway components activate son enhancer trap expression in wing cells. son enhancer trap expression is regulated positively by wingless, and negatively by cut in boundary cells. Ectopic Son protein induces wingless and cut expression in wing discs. We hypothesize that there is positive feedback regulation of son by wingless, and negative regulation by cut at the dorsal-ventral boundary during wing development.
进行了P因子增强子陷阱筛选,以鉴定参与果蝇背腹边界形成的基因。通过son(2205)增强子陷阱插入鉴定出Notch的儿子(son)基因,这是一种部分功能丧失突变。基于son(2205)突变体表型以及与Notch和无翅突变的遗传相互作用,我们得出结论:son参与翅膀发育,并在翅膀的背腹边界的Notch信号通路中发挥作用。Notch信号通路成分激活翅膀细胞中的son增强子陷阱表达。son增强子陷阱表达在边界细胞中受到无翅的正向调节和cut的负向调节。异位Son蛋白在翅盘中诱导无翅和cut表达。我们推测,在翅膀发育过程中,son在背腹边界受到无翅的正反馈调节和cut的负调节。