Neumann C J, Cohen S M
European Molecular Biology Laboratory, Heidelberg, Germany.
Development. 1996 Nov;122(11):3477-85. doi: 10.1242/dev.122.11.3477.
Short-range interaction between dorsal and ventral cells establishes an organizing center at the dorsal/ventral compartment boundary that controls growth and patterning of the wing. We report here that the dorsal/ventral organiser is built though a hierarchy of regulatory interactions involving the Notch and wingless signal transduction pathways and the vestigial gene. wingless and vestigial are activated in cells adjacent to the dorsal/ventral boundary by a Notch-dependent signal. vestigial is initially expressed under control of an early dorsal/ventral boundary enhancer that does not depend on wingless activity. Similarly, activation of wingless does not require vestigial function, showing that wingless and vestigial are parallel targets of the Notch pathway. Subsequently, vestigial is expressed in a broad domain that fills the wing pouch. This second phase of vestigial expression depends on Wingless function in cells at the dorsal/ventral boundary. In addition, the Notch and Wingless pathways act synergistically to regulate expression of cut in cells at the dorsal/ventral boundary. Thus Wingless can act locally, in combination with Notch, to specify cell fates, as well as at a distance to control vestigial expression. These results suggest that secreted Wingless protein mediates both long-range and short-range patterning activities of the dorsal/ventral boundary.
背侧和腹侧细胞之间的短程相互作用在背/腹区室边界处建立了一个组织中心，该中心控制翅膀的生长和模式形成。我们在此报告，背/腹组织者是通过涉及Notch和无翅信号转导途径以及残翅基因的调控相互作用层次构建而成的。无翅基因和残翅基因在与背/腹边界相邻的细胞中被Notch依赖性信号激活。残翅基因最初在一个不依赖无翅基因活性的早期背/腹边界增强子的控制下表达。同样，无翅基因的激活不需要残翅基因的功能，这表明无翅基因和残翅基因是Notch途径的平行靶点。随后，残翅基因在填充翅芽的广泛区域中表达。残翅基因表达的第二阶段取决于背/腹边界处细胞中的无翅基因功能。此外，Notch和无翅基因途径协同作用以调节背/腹边界处细胞中cut基因的表达。因此，无翅基因可以与Notch结合在局部发挥作用，以确定细胞命运，也可以在远距离发挥作用以控制残翅基因的表达。这些结果表明，分泌的无翅蛋白介导了背/腹边界的长程和短程模式形成活动。
