Li Baoe, Liu Xuanyong, Cao Cong, Dong Yuqi, Wang Zheng, Ding Chuanxian
Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
J Biomed Mater Res A. 2008 Oct;87(1):84-90. doi: 10.1002/jbm.a.31776.
In this work, gentamicin loaded collagen was grafted on the surface of plasma sprayed wollastonite coatings to obtain an implant having excellent bioactivity and cytocompatibility as well as antibacterial property. The bioactivity and cytocompatibility of the wollastonite coatings grafting gentamicin loaded collagen were examined by simulated body fluid (SBF) soaking test and in vitro cell culture test. The release rate of gentamicin from collagen was measured using UV spectrophotometer in phosphate-buffered saline (PBS) and the antibacterial activity against Staphylococcus aureus (S. aureus) was evaluated by zone of inhibition test and bacterial counting method. The results showed that a composite layer with collagen and silicon-rich particles was formed on the surface of the coating after the graft of collagen. The grafted collagen layer mixed with silicon-rich particles could induce the precipitation of apatite after soaking in SBF for 14 days and improved the cellular proliferation on wollastonite coatings. The release of gentamicin from the collagen layer sustained 11 days in PBS and effectively inhibited the growth of S. aureus. In conclusion, the wollastonite coating grafting gentamicin loaded collagen had excellent bioactivity and cytocompatibility as well as good antibacterial properties, which would be beneficial for the long term stability and surgical success rate of implants.
在本研究中，将负载庆大霉素的胶原蛋白接枝到等离子喷涂硅灰石涂层表面，以获得具有优异生物活性、细胞相容性和抗菌性能的植入物。通过模拟体液（SBF）浸泡试验和体外细胞培养试验，对接枝负载庆大霉素胶原蛋白的硅灰石涂层的生物活性和细胞相容性进行了研究。使用紫外分光光度计在磷酸盐缓冲盐水（PBS）中测定庆大霉素从胶原蛋白中的释放速率，并通过抑菌圈试验和细菌计数法评估其对金黄色葡萄球菌（S. aureus）的抗菌活性。结果表明，接枝胶原蛋白后，涂层表面形成了含有胶原蛋白和富硅颗粒的复合层。接枝的胶原蛋白层与富硅颗粒混合，在SBF中浸泡14天后可诱导磷灰石沉淀，并改善了硅灰石涂层上的细胞增殖。庆大霉素从胶原蛋白层在PBS中的释放持续11天，并有效抑制了金黄色葡萄球菌的生长。总之，接枝负载庆大霉素胶原蛋白的硅灰石涂层具有优异的生物活性和细胞相容性以及良好的抗菌性能，这将有利于植入物的长期稳定性和手术成功率。
