Li Baoe, Liu Xuanyong, Cao Cong, Ding Chuanxian
Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China.
Department of Orthopaedics, Renji Hospital, College of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
J Biomed Mater Res A. 2007 Dec 15;83(4):923-930. doi: 10.1002/jbm.a.31414.
In this article, the plasma sprayed titania coatings were treated by grafting pure and gentamicin loaded collagen to improve the biocompatibility and antibacterial activity. The biocompatibility of the titania coating grafting collagen was evaluated by in vitro cell culturing test. The release rate of gentamicin from collagen was measured in tris-HCl buffer using UV spectrophotometer, and the antibacterial activity of the titania coating with gentamicin against Staphylococcus aureus was examined using the zone of inhibition test. The results showed that collagen was successfully grafted on the surface of titania coatings treated by sulfuric acid. The in vitro cell culturing test revealed that collagen significantly improved the cell adhesion and proliferation on the surface of titania coatings. The gentamicin loaded in collagen matrix could retain a sustained release in tris-HCl for 30 days, which was efficient to protect against the postoperative infection caused by S. aureus. The results indicated that the plasma sprayed titania coating grafting collagen and gentamicin would have the antibacterial activity together with the biocompatibility, which might be beneficial for the long term stability and surgical success rate of implants.
在本文中，通过接枝纯胶原蛋白和负载庆大霉素的胶原蛋白对等离子喷涂二氧化钛涂层进行处理，以提高其生物相容性和抗菌活性。通过体外细胞培养试验评估了接枝胶原蛋白的二氧化钛涂层的生物相容性。使用紫外分光光度计在三羟甲基氨基甲烷盐酸盐（Tris-HCl）缓冲液中测定庆大霉素从胶原蛋白中的释放速率，并使用抑菌圈试验检测负载庆大霉素的二氧化钛涂层对金黄色葡萄球菌的抗菌活性。结果表明，胶原蛋白成功接枝到了经硫酸处理的二氧化钛涂层表面。体外细胞培养试验表明，胶原蛋白显著改善了二氧化钛涂层表面的细胞黏附和增殖。负载在胶原蛋白基质中的庆大霉素在Tris-HCl中可实现30天的持续释放，这对于预防由金黄色葡萄球菌引起的术后感染有效。结果表明，接枝胶原蛋白和庆大霉素的等离子喷涂二氧化钛涂层将同时具有抗菌活性和生物相容性，这可能有利于植入物的长期稳定性和手术成功率。
