大多数秀丽隐杆线虫的微小RNA对发育或生存能力并非不可或缺。
Most Caenorhabditis elegans microRNAs are individually not essential for development or viability.
作者信息
Miska Eric A, Alvarez-Saavedra Ezequiel, Abbott Allison L, Lau Nelson C, Hellman Andrew B, McGonagle Shannon M, Bartel David P, Ambros Victor R, Horvitz H Robert
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
出版信息
PLoS Genet. 2007 Dec;3(12):e215. doi: 10.1371/journal.pgen.0030215.
MicroRNAs (miRNAs), a large class of short noncoding RNAs found in many plants and animals, often act to post-transcriptionally inhibit gene expression. We report the generation of deletion mutations in 87 miRNA genes in Caenorhabditis elegans, expanding the number of mutated miRNA genes to 95, or 83% of known C. elegans miRNAs. We find that the majority of miRNAs are not essential for the viability or development of C. elegans, and mutations in most miRNA genes do not result in grossly abnormal phenotypes. These observations are consistent with the hypothesis that there is significant functional redundancy among miRNAs or among gene pathways regulated by miRNAs. This study represents the first comprehensive genetic analysis of miRNA function in any organism and provides a unique, permanent resource for the systematic study of miRNAs.
微小RNA（miRNA）是在许多动植物中发现的一大类短链非编码RNA，常作用于转录后抑制基因表达。我们报告了秀丽隐杆线虫中87个miRNA基因缺失突变的产生，使突变的miRNA基因数量增加到95个，占已知秀丽隐杆线虫miRNA的83%。我们发现，大多数miRNA对秀丽隐杆线虫的生存能力或发育并非必不可少，大多数miRNA基因的突变不会导致明显异常的表型。这些观察结果与miRNA之间或受miRNA调控的基因通路之间存在显著功能冗余的假设一致。本研究代表了对任何生物体中miRNA功能的首次全面遗传分析，并为miRNA的系统研究提供了独特的永久性资源。
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