Forslind B, Malmqvist K G, Pallon J
Department of Medical Biophysics, Karolinska Institute, Stockholm, Sweden.
Scanning Microsc. 1991 Sep;5(3):877-84.
Proton induced X-ray emission (PIXE) analysis is a comparatively new member of the family of spectrographic methods. In the last decade PIXE techniques have been applied to biological problems with great success. This review gives a condensed presentation of recent developments in biological (medical, zoological, and botanical) applications of PIXE analysis with special focus on factors which commonly influence the results, such as calibration, contamination, and preparation. The great advantage of PIXE analysis in studying physiologically important trace elements such as Zn, Mg, Fe, and Cu is underlined. Elemental mapping not only allows quantitative elemental analysis, but can also demonstrate the important differences in the morphological distributions of elements by comparing normal and pathological tissue.
质子激发X射线发射（PIXE）分析是光谱分析方法家族中相对较新的成员。在过去十年中，PIXE技术已成功应用于生物学问题。本综述简要介绍了PIXE分析在生物学（医学、动物学和植物学）应用中的最新进展，特别关注通常影响结果的因素，如校准、污染和样品制备。强调了PIXE分析在研究生理上重要的微量元素（如锌、镁、铁和铜）方面的巨大优势。元素映射不仅可以进行定量元素分析，还可以通过比较正常组织和病理组织来展示元素形态分布的重要差异。
